Immunological control of murine gammaherpesvirus infection is independent of perforin

EJ Usherwood, JW Brooks, SR Sarawar, RD Cardin, WD Young, DJ Allen, PC Doherty and AA Nash Department of Veterinary Pathology, University of Edinburgh, Summerhall, UK. Perforin-mediated cytotoxic T cell killing has been suggested to be of importance in the control of noncytopathic virus infections,...

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Bibliographic Details
Published in:Journal of general virology 1997-08, Vol.78 (8), p.2025-2030
Main Authors: Usherwood, EJ, Brooks, JW, Sarawar, SR, Cardin, RD, Young, WD, Allen, DJ, Doherty, PC, Nash, AA
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
CD8-Positive T-Lymphocytes - immunology
Cell Line
Cricetinae
Cytokines - biosynthesis
Gammaherpesvirinae - immunology
Gammaherpesvirinae - pathogenicity
Herpesviridae Infections - immunology
Interferon-gamma - biosynthesis
Interleukin-10 - biosynthesis
Interleukin-6 - biosynthesis
Lung - immunology
Lung - virology
Lymph Nodes - immunology
Membrane Glycoproteins - deficiency
Membrane Glycoproteins - physiology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Perforin
Pore Forming Cytotoxic Proteins
Spleen - immunology
Spleen - virology
T-Lymphocytes, Cytotoxic - immunology
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Summary:EJ Usherwood, JW Brooks, SR Sarawar, RD Cardin, WD Young, DJ Allen, PC Doherty and AA Nash Department of Veterinary Pathology, University of Edinburgh, Summerhall, UK. Perforin-mediated cytotoxic T cell killing has been suggested to be of importance in the control of noncytopathic virus infections, based on studies with lymphocytic choriomeningitis virus (LCMV). We examined the role of perforin in a mouse model of gammaherpesvirus infection using transgenic perforin-deficient mice. Previous work from this laboratory has shown that CD8 T cells are essential for the resolution of the acute lung infection and control of latently infected B cells in murine gamma-herpesvirus 68 infection. The absence of perforin did not significantly affect the kinetics of either the lytic lung infection or the latent spleen infection. Lymphocytes from both perforin-deficient and control mice secreted comparable levels of IFN-gamma, IL-10 and IL- 6. In addition, lymphocytes from both strains had similar levels of CD3epsilon-dependent cytotoxic activity in the spleen, draining lymph nodes and bronchoalveolar lavage. These data indicate that the lack of perforin has little affect on the ability of mice to control an experimental gammaherpesvirus infection.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-78-8-2025