Cloning and characterization of actin depolymerizing factor from Toxoplasma gondii

We determined the predicted amino acid sequence of actin depolymerizing factor (ADF) from Toxoplasma gondii by sequencing the full-length cDNA. T. gondii ADF consists of 118 amino acids (calculated molecular weight 13 400) and shares a high degree of sequence similarity to other low molecular weight...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1997-09, Vol.88 (1), p.43-52
Main Authors: Allen, M.Leah, Dobrowolski, Janice M, Muller, Hayyah, Sibley, L.David, Mansour, Tag E
Format: Article
Language:English
Subjects:
Actin depolymerizing factor
Actin Depolymerizing Factors
Actins - metabolism
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Cytoskeleton
Destrin
DNA Primers - genetics
DNA, Complementary - genetics
DNA, Protozoan - genetics
Genes, Protozoan
Immunohistochemistry
Microfilament Proteins - genetics
Microfilament Proteins - isolation & purification
Microfilament Proteins - metabolism
Molecular Sequence Data
Polymerase Chain Reaction
Protozoan Proteins - genetics
Protozoan Proteins - isolation & purification
Protozoan Proteins - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
Recombinant Proteins - metabolism
Toxoplasma - genetics
Toxoplasma - metabolism
Toxoplasma gondii
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Summary:We determined the predicted amino acid sequence of actin depolymerizing factor (ADF) from Toxoplasma gondii by sequencing the full-length cDNA. T. gondii ADF consists of 118 amino acids (calculated molecular weight 13 400) and shares a high degree of sequence similarity to other low molecular weight actin monomer sequestering proteins, especially Acanthamoeba actophorin, plant ADFs and yeast and vertebrate cofilin. ADF from T. gondii is smaller and does not contain a nuclear localization sequence like the related vertebrate proteins. Southern blot analysis indicates that T. gondii ADF is a single-copy gene. Homogeneous recombinant T. gondii ADF purified from E. coli is active in binding actin monomers and depolymerizing F-actin. Localization of ADF by immunofluorescence and immuno-electron microscopy indicates ADF is scattered throughout the cytoplasm and prominently localized beneath the plasma membrane in T. gondii.
ISSN:0166-6851
1872-9428
DOI:10.1016/S0166-6851(97)00069-8