Potential Involvement of Both Type I and Type II Mechanisms in M13 Virus Inactivation by Methylene Blue Photosensitization

We have investigated the mechanism of virus photoinactivation with methylene blue (MB) by conducting deuterium oxide (D2O), azide ion (N3‐) and oxygen‐dependent, studies. Inactivation of M13 bacteriophage and singlet oxygen (1O2) generation by MB photosensitization were irradiation dose dependent. I...

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Bibliographic Details
Published in:Photochemistry and photobiology 1997-08, Vol.66 (2), p.204-208
Main Authors: Abe, H., Ikebuchi, K., Wagner, S. J., Kuwabara, M., Kamo, N., Sekiguchi, S.
Format: Article
Language:English
Subjects:
Antiviral Agents - pharmacology
Bacteriophage M13 - drug effects
Deuterium - metabolism
Methylene Blue - pharmacology
Nitrogen - metabolism
Oxygen - metabolism
Photochemistry
Photosensitizing Agents - pharmacology
Singlet Oxygen
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Summary:We have investigated the mechanism of virus photoinactivation with methylene blue (MB) by conducting deuterium oxide (D2O), azide ion (N3‐) and oxygen‐dependent, studies. Inactivation of M13 bacteriophage and singlet oxygen (1O2) generation by MB photosensitization were irradiation dose dependent. Inactivation of M13 was enhanced by D2O and inhibited by N3‐, suggesting that 1O2 participates in M13 inactivation by MB photosensitization. However, N3‐ did not inhibit M13 inactivation completely. On the other hand, deoxygenating the reaction solution still caused 52‐67% of M13 inactivation observed in the presence of oxygen. These results suggest that 102‐mediated (Type II) and sensitizer‐mediated (Type I) reactions may both play roles in M13 inactivation by MB photosensitization.
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.1997.tb08644.x