Carcinoembryonic antigen induces signal transduction in Kupffer cells

Carcinoembryonic antigen (CEA), an iptercellular adhesion molecule and a mediator of hepatic metastasis, is processed by an 80 kDa receptor on murine and human Kupffer cells in the liver. Activation of rat Kupffer cells in vitro by CEA via the 80 kDa receptor produced cytokines IL-1α and TNF-α which...

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Bibliographic Details
Published in:Cancer letters 1997-09, Vol.118 (1), p.1-6
Main Authors: Gangopadhyay, Aniruddha, Lazure, Donald A., Thomas, Peter
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carcinoembryonic antigen
Carcinoembryonic Antigen - isolation & purification
Carcinoembryonic Antigen - pharmacology
Carcinogenesis, carcinogens and anticarcinogens
Carrier Proteins - chemistry
Carrier Proteins - physiology
Cells, Cultured
Colorectal Neoplasms - chemistry
General aspects
Host-tumor relations. Immunology. Biological markers
Humans
Interleukin-1 - biosynthesis
Kinetics
Kupffer cell
Kupffer Cells - drug effects
Kupffer Cells - immunology
Kupffer Cells - physiology
Liver Neoplasms - chemistry
Liver Neoplasms - secondary
Male
Medical sciences
Mice
Molecular Weight
Phosphorylation
Phosphotyrosine - analysis
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface
Signal transduction
Signal Transduction - drug effects
Tumor Necrosis Factor-alpha - biosynthesis
Tumors
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Summary:Carcinoembryonic antigen (CEA), an iptercellular adhesion molecule and a mediator of hepatic metastasis, is processed by an 80 kDa receptor on murine and human Kupffer cells in the liver. Activation of rat Kupffer cells in vitro by CEA via the 80 kDa receptor produced cytokines IL-1α and TNF-α which involved tyrosine phosphorylation. The peak response of TNF-α was 5.6 times greater than the corresponding IL-1α response and was associated with enhanced tyrosine phosphorylation of 108 and 125 kDa proteins. Lipopolysaccharide (LPS) treatment, on the other hand, phosphorylated two major proteins with MW of 93 and 119 kDa associated with the loss of phosphorylation from a 125 kDa protein. Results demonstrate that CEA-induced IL-1α and TNF-α production involves tyrosine phosphorylation and the signaling in CEA treated cells is different than that seen with LPS stimulation.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(97)00216-4