A chemically transformed rat fibroblast cell line expresses high levels of oncomodulin

Chemically (by N-methyl- N-nitro- N-nitrosoguanidine) treated rat fibroblasts (T14c) exhibited growth characteristics and a morphology typical for transformed cells and markedly different from untreated, parental cells. In contrast to untransformed cells, T14c fibroblasts produced significant levels...

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Bibliographic Details
Published in:FEBS Letters 1989-11, Vol.257 (2), p.307-310
Main Authors: Sommer, E.W., Blum, J.K., Berger, M.C., Berchtold, M.W.
Format: Article
Language:English
Subjects:
Animal cells
Animals
Biological and medical sciences
Blotting, Northern
Ca 2+-binding protein
Ca2+-binding protein
Calcium-Binding Proteins - genetics
Calmodulin
Calmodulin - metabolism
CAM, calmodulin
Cell cultures. Hybridization. Fusion
Cell Division
Cell Transformation, Neoplastic - genetics
Chemical transformation
Fibroblast
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Neoplastic
Liver Neoplasms, Experimental - genetics
LTR, long terminal repeat
MNNG, N-methyl-N-nitro-N-nitrosoguanidine
Molecular and cellular biology
OM, oncomudolin
Oncomodulin
Rats
RNA, Messenger - genetics
RNA, Neoplasm - genetics
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Summary:Chemically (by N-methyl- N-nitro- N-nitrosoguanidine) treated rat fibroblasts (T14c) exhibited growth characteristics and a morphology typical for transformed cells and markedly different from untreated, parental cells. In contrast to untransformed cells, T14c fibroblasts produced significant levels of oncomodulin mRNA as analyzed on Northern blots even when compared to rat Morris hepatomas, the richest source of oncomodulin known so far. The levels of transcripts for both calmodulin and oncomodulin in T14c cells were higher in log phase growth as compared to confluent stages.The T14c model system may be useful in the elucidation of mechanisms involved in the regulation of oncomodulin synthesis.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)81558-3