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Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp f...
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| Published in: | Gene 1997-09, Vol.197 (1), p.289-293 |
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| Main Authors: | , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c391t-f7ced0b3847933eaac75c66f6d6003b8fcfe688ba1b537a4c98e36e0928f6cbc3 |
| container_end_page | 293 |
| container_issue | 1 |
| container_start_page | 289 |
| container_title | Gene |
| container_volume | 197 |
| creator | Akira, Toko Komatsu, Masami Nango, Rieko Tomooka, Akiko Kimiko Konaka Yamauchi, Masatake Kitamura, Yukihiko Nomura, Shintaro Tsukamoto, Ikuyo |
| description | The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928
bp containing a coding region of 1779
bp for a 592-amino acid polypeptide (
M
r=64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24
h after partial (70%) hepatectomy. |
| doi_str_mv | 10.1016/S0378-1119(97)00273-4 |
| format | article |
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bp containing a coding region of 1779
bp for a 592-amino acid polypeptide (
M
r=64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24
h after partial (70%) hepatectomy.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(97)00273-4</identifier><identifier>PMID: 9332377</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>10-Formyltetrahydrofolate ; Amino Acid Sequence ; Animals ; Bifunctional protein ; Cloning, Molecular ; DNA, Complementary - genetics ; Escherichia coli - genetics ; Gene Expression Regulation, Enzymologic - genetics ; Hepatectomy ; Hydroxymethyl and Formyl Transferases - genetics ; Liver - physiology ; Liver Regeneration - genetics ; Molecular Sequence Data ; Nucleotide Deaminases - genetics ; Phosphoribosylaminoimidazolecarboxamide Formyltransferase ; purH gene ; Purine synthesis ; Rats ; Recombinant Proteins ; RNA, Messenger - analysis ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid</subject><ispartof>Gene, 1997-09, Vol.197 (1), p.289-293</ispartof><rights>1997 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-f7ced0b3847933eaac75c66f6d6003b8fcfe688ba1b537a4c98e36e0928f6cbc3</citedby><cites>FETCH-LOGICAL-c391t-f7ced0b3847933eaac75c66f6d6003b8fcfe688ba1b537a4c98e36e0928f6cbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9332377$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akira, Toko</creatorcontrib><creatorcontrib>Komatsu, Masami</creatorcontrib><creatorcontrib>Nango, Rieko</creatorcontrib><creatorcontrib>Tomooka, Akiko</creatorcontrib><creatorcontrib>Kimiko Konaka</creatorcontrib><creatorcontrib>Yamauchi, Masatake</creatorcontrib><creatorcontrib>Kitamura, Yukihiko</creatorcontrib><creatorcontrib>Nomura, Shintaro</creatorcontrib><creatorcontrib>Tsukamoto, Ikuyo</creatorcontrib><title>Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase</title><title>Gene</title><addtitle>Gene</addtitle><description>The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928
bp containing a coding region of 1779
bp for a 592-amino acid polypeptide (
M
r=64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24
h after partial (70%) hepatectomy.</description><subject>10-Formyltetrahydrofolate</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bifunctional protein</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Gene Expression Regulation, Enzymologic - genetics</subject><subject>Hepatectomy</subject><subject>Hydroxymethyl and Formyl Transferases - genetics</subject><subject>Liver - physiology</subject><subject>Liver Regeneration - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleotide Deaminases - genetics</subject><subject>Phosphoribosylaminoimidazolecarboxamide Formyltransferase</subject><subject>purH gene</subject><subject>Purine synthesis</subject><subject>Rats</subject><subject>Recombinant Proteins</subject><subject>RNA, Messenger - analysis</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhi0EKtvCI1TyCcEh1I4T2zmhqqVQqQUk4Gw54zEYJfFiJ6jLI_DUON1Vr_XF8szv_9fMR8gpZ2854_LsKxNKV5zz7nWn3jBWK1E1T8iGa9VVjAn9lGweJM_Jcc6_WDltWx-Ro06IWii1If9u44CwDDZRGOIUph_UTo7i3TZhziFONHpqabIzhctP5xQniG5VtZUdwxTDGJz9WzyqpgKb-nhXyg5pCn2cFhgwzuvTxzTuhjnZKXtMNuPZ9e0XCruS-XPnUhxK6QV55u2Q8eXhPiHfr95_u_hY3Xz-cH1xflOB6PhceQXoWC90o8oUaC2oFqT00skyda89eJRa95b3rVC2gU6jkMi6WnsJPYgT8mrvu03x94J5NmPIgMNgJ4xLNqutbEXzqJBL1ipdd0XY7oWQYs4JvdmmMNq0M5yZFZa5h2VWEqZT5h6WWQNODwFLP6J7-HWgU_rv9n0s6_gTMJkMoRBAFxLCbFwMjyT8By8Kp00</recordid><startdate>19970915</startdate><enddate>19970915</enddate><creator>Akira, Toko</creator><creator>Komatsu, Masami</creator><creator>Nango, Rieko</creator><creator>Tomooka, Akiko</creator><creator>Kimiko Konaka</creator><creator>Yamauchi, Masatake</creator><creator>Kitamura, Yukihiko</creator><creator>Nomura, Shintaro</creator><creator>Tsukamoto, Ikuyo</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19970915</creationdate><title>Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase</title><author>Akira, Toko ; Komatsu, Masami ; Nango, Rieko ; Tomooka, Akiko ; Kimiko Konaka ; Yamauchi, Masatake ; Kitamura, Yukihiko ; Nomura, Shintaro ; Tsukamoto, Ikuyo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-f7ced0b3847933eaac75c66f6d6003b8fcfe688ba1b537a4c98e36e0928f6cbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>10-Formyltetrahydrofolate</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bifunctional protein</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Gene Expression Regulation, Enzymologic - genetics</topic><topic>Hepatectomy</topic><topic>Hydroxymethyl and Formyl Transferases - genetics</topic><topic>Liver - physiology</topic><topic>Liver Regeneration - genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleotide Deaminases - genetics</topic><topic>Phosphoribosylaminoimidazolecarboxamide Formyltransferase</topic><topic>purH gene</topic><topic>Purine synthesis</topic><topic>Rats</topic><topic>Recombinant Proteins</topic><topic>RNA, Messenger - analysis</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akira, Toko</creatorcontrib><creatorcontrib>Komatsu, Masami</creatorcontrib><creatorcontrib>Nango, Rieko</creatorcontrib><creatorcontrib>Tomooka, Akiko</creatorcontrib><creatorcontrib>Kimiko Konaka</creatorcontrib><creatorcontrib>Yamauchi, Masatake</creatorcontrib><creatorcontrib>Kitamura, Yukihiko</creatorcontrib><creatorcontrib>Nomura, Shintaro</creatorcontrib><creatorcontrib>Tsukamoto, Ikuyo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akira, Toko</au><au>Komatsu, Masami</au><au>Nango, Rieko</au><au>Tomooka, Akiko</au><au>Kimiko Konaka</au><au>Yamauchi, Masatake</au><au>Kitamura, Yukihiko</au><au>Nomura, Shintaro</au><au>Tsukamoto, Ikuyo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1997-09-15</date><risdate>1997</risdate><volume>197</volume><issue>1</issue><spage>289</spage><epage>293</epage><pages>289-293</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928
bp containing a coding region of 1779
bp for a 592-amino acid polypeptide (
M
r=64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24
h after partial (70%) hepatectomy.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9332377</pmid><doi>10.1016/S0378-1119(97)00273-4</doi><tpages>5</tpages></addata></record> |
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| ispartof | Gene, 1997-09, Vol.197 (1), p.289-293 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79336534 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | 10-Formyltetrahydrofolate Amino Acid Sequence Animals Bifunctional protein Cloning, Molecular DNA, Complementary - genetics Escherichia coli - genetics Gene Expression Regulation, Enzymologic - genetics Hepatectomy Hydroxymethyl and Formyl Transferases - genetics Liver - physiology Liver Regeneration - genetics Molecular Sequence Data Nucleotide Deaminases - genetics Phosphoribosylaminoimidazolecarboxamide Formyltransferase purH gene Purine synthesis Rats Recombinant Proteins RNA, Messenger - analysis Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid |
| title | Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase |
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