Recombinant core particles of hepatitis B virus exposing foreign antigenic determinants on their surface

Insertion of foreign oligopeptide sequences (40–50 amino acids in length) into the Pro 144 position of hepatitis B core antigen (HBcAg) leads to the formation of chimeric capsids in Escherichia coli cells. These capsids are morphologically and immunogically similar to native HBcAg, but expose the in...

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Bibliographic Details
Published in:FEBS letters 1989-12, Vol.259 (1), p.121-124
Main Authors: Borisova, G.P., Berzins, I., Pushko, P.M., Pumpen, P., Gren, E.J., Tsibinogin, V.V., Loseva, V., Ose, V., Ulrich, R., Siakkou, H., Rosenthal, H.A.
Format: Article
Language:English
Subjects:
AIDS/HIV
Antigenic determinant
Antigens, Viral - genetics
Biological and medical sciences
Bovine leukemia virus envelope protein gp51
Capsid - immunology
DNA, Recombinant
Epitopes
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetic Vectors
Hepatitis B Antigens - genetics
Hepatitis B core antigen
Hepatitis B Core Antigens - genetics
Hepatitis B Core Antigens - immunology
Hepatitis B virus - genetics
Hepatitis B virus - immunology
Hepatitis B virus - ultrastructure
HIV Envelope Protein gp41 - genetics
HIV Envelope Protein gp41 - immunology
Human immunodeficiency virus-1 transmembrane protein gp41
Immunohistochemistry
Leukemia Virus, Bovine - genetics
Leukemia Virus, Bovine - immunology
Microbiology
Molecular Sequence Data
Morphology, structure, chemical composition, physicochemical properties
PreS region
Vaccines, Synthetic
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Virology
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Summary:Insertion of foreign oligopeptide sequences (40–50 amino acids in length) into the Pro 144 position of hepatitis B core antigen (HBcAg) leads to the formation of chimeric capsids in Escherichia coli cells. These capsids are morphologically and immunogically similar to native HBcAg, but expose the inserted oligopeptides on their outer surface and exhibit antigenic and immunogenic characteristics of the latter. As a source of model antigenic determinants, the appropriate DNA copies excised from cloned viral genes such as the pre-S region of hepatitis B virus, the transmembrane protein gp41 of human immunodeficiency virus 1 and the envelope protein gp51 of bovine leukemia virus have been used. The localization of the inserted antigenic determinants on the surface of chimeric capsids does not depend on the presence or absence of the arginine-rich, 39 amino acid-long C terminus of HBcAg.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)81509-1