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Effects of serum from preeclamptic women on prostacyclin production by human endothelial cells

There is growing evidence that proteinuric hypertension of pregnancy (preeclampsia) is associated with endothelial dysfunction. The aim of this study was to evaluate the effects of serum from preeclamptic patients on basal and agonist‐stimulated prostacyclin production by human umbilical vein endoth...

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Published in:The Journal of maternal-fetal medicine 1997-09, Vol.6 (5), p.249-253
Main Authors: Winn, Hung N., Todd, Herbert M., Amon, Erol, Al‐Malt, Ahmed, Molnár, Miklós, Hertelendy, Frank
Format: Article
Language:English
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Summary:There is growing evidence that proteinuric hypertension of pregnancy (preeclampsia) is associated with endothelial dysfunction. The aim of this study was to evaluate the effects of serum from preeclamptic patients on basal and agonist‐stimulated prostacyclin production by human umbilical vein endothelial cells (HUVEC) in culture and to compare these to the effects of serum from normal pregnant and nonpregnant women. During a 24 h incubation of HUVEC with 20% preeclampsia serum, baseline prostacyclin output was significantly (P < 0.01) increased over the control groups. However, this response was attenuated by extending the exposure to 72 h. Histamine, thrombin and the calcium ionophore, A23187, all acutely increased prostacyclin production, but the increase relative to baseline levels was greatest in HUVEC preincubated for 24 h in normal serum and was smallest when cells were preincubated in preeclampsia serum. We conclude that 1) preeclampsia serum transiently promotes prostacyclin production in HUVEC derived from normal pregnancies, and 2) the relative increase in response to agonists is reduced by preeclampsia serum, compared to normal pregnancy sera. J. Matern.–Fetal Med. 6:249–253, 1997. © 1997 Wiley‐Liss, Inc.
ISSN:1057-0802
1520-6661
DOI:10.1002/(SICI)1520-6661(199709/10)6:5<249::AID-MFM1>3.0.CO;2-G