Mechanical and energy characteristics during shortening in isolated type-1 muscle fibres from Xenopus laevis studied at maximal and submaximal activation

• Published in: Pflügers Archiv 1997-12, Vol.435 (1), p.145-150
• Main Authors: Buschman, H P, Linari, M, Elzinga, G, Woledge, R C
• Format: Article
• Language: English
• Subjects: Actomyosin - metabolism; Animals; Biomechanical Phenomena; Efficiency; Electric Stimulation; Energy Metabolism; Female; Isometric Contraction; Muscle Contraction; Muscle Fibers, Slow-Twitch - physiology; Muscular system; Space life sciences; Xenopus laevis
• ISSN: 0031-6768; 1432-2013
• DOI: 10.1007/s004240050494

The mechanical and energy characteristics of isolated fast-twitch muscle fibres (type 1) of Xenopus laevis in isometric- and isovelocity contractions were measured at 20 degrees C. The fibres were stimulated at either 60 Hz or 20 Hz to produce contractions at different levels of activation. The high stimulation frequency gave fused contractions, while at the low stimulation frequency tension fluctuated. When maximum isometric force had been reached, the fibres were shortened by 10% of the fibre length at different velocities. At 60 Hz stimulation during shortening the rate of heat production increased above the isometric rate of heat production. At 20 Hz stimulation during shortening, however, the rate of heat production was not different from the isometric rate of heat production. Mechanical efficiency was the same at the high and low level of activation. The actomyosin efficiency (i.e. the mechanical efficiency corrected for "activation heat") was highest at the low level of activation. We conclude that in fast-twitch muscle fibres from X. laevis, actomyosin efficiency is highest for partially activated muscle. From a comparison of the present results with those obtained from a study of slow-twitch muscle fibres presented earlier, it is concluded that fast-twitch muscle fibres are less efficient than slow-twitch muscle fibres.