Loading…
Comparison of Serotonin and Dopamine Release in Substantia Nigra and Ventral Tegmental Area: Region and Species Differences
: In this study, we compare the electrically evoked, somatodendritic release of dopamine (DA) with axonal release of serotonin (5‐HT) in the substantia nigra (SN) and ventral tegmental area (VTA) in vitro by using fast‐scan cyclic voltammetry with carbon‐fibre microelectrodes. Furthermore, we have e...
Saved in:
| Published in: | Journal of neurochemistry 1997-12, Vol.69 (6), p.2378-2386 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c4908-34b1ea58bbdf16820e0d99d9b77de445e72c25aee60a58f0c8c23717825af99c3 |
|---|---|
| cites | |
| container_end_page | 2386 |
| container_issue | 6 |
| container_start_page | 2378 |
| container_title | Journal of neurochemistry |
| container_volume | 69 |
| creator | Cragg, S. J. Hawkey, C. R. Greenfield, S. A. |
| description | : In this study, we compare the electrically evoked, somatodendritic release of dopamine (DA) with axonal release of serotonin (5‐HT) in the substantia nigra (SN) and ventral tegmental area (VTA) in vitro by using fast‐scan cyclic voltammetry with carbon‐fibre microelectrodes. Furthermore, we have examined transmitter release in these regions in guinea‐pig compared with rat. Somatodendritic DA was released, as shown previously, in guinea‐pig VTA, SN pars compacta (SNc), and occasionally in SN pars reticulata (SNr). 5‐HT was rarely released, except in SNr, where nonetheless it only contributed to |
| doi_str_mv | 10.1046/j.1471-4159.1997.69062378.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79427788</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16387890</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4908-34b1ea58bbdf16820e0d99d9b77de445e72c25aee60a58f0c8c23717825af99c3</originalsourceid><addsrcrecordid>eNqVkV1v0zAUhi0EGmXwE5AigbhLsB3HH3A1tYwPTUOig1vLcU4qV4md2anYtD-PS7veIq58fN7nPcfyi9AbgiuCGX-_rQgTpGSkURVRSlRcYU5rIau7J2hx0p6iBcaUljVm9Dl6kdIWY8IZJ2foTNWi4Vwt0MMyjJOJLgVfhL5YQwxz8M4XxnfFKkxmdB6KHzCASVDk_nrXptn42Zni2m2i-Qv-Aj9HMxQ3sBlzmauLCOZD9m1cOMxaT2AdpGLl-h4ieAvpJXrWmyHBq-N5jn5efrpZfimvvn_-ury4Ki1TWJY1awmYRrZt1xMuKQbcKdWpVogOGGtAUEsbA8Bxpnpspc2fQYTMzV4pW5-jd4e5Uwy3O0izHl2yMAzGQ9glLRSjQkj5T5DwWgqpcAY_HkAbQ0oRej1FN5p4rwnW-4z0Vu9z0Psc9D4j_ZiRvsvu18c1u3aE7uQ9hpL1t0fdJGuGPhpvXTphFEvV8DpjqwP22w1w_z8v0N-ul4-3-g_RNK9A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16387890</pqid></control><display><type>article</type><title>Comparison of Serotonin and Dopamine Release in Substantia Nigra and Ventral Tegmental Area: Region and Species Differences</title><source>Full-Text Journals in Chemistry (Open access)</source><source>Wiley-Blackwell Read & Publish Collection</source><creator>Cragg, S. J. ; Hawkey, C. R. ; Greenfield, S. A.</creator><creatorcontrib>Cragg, S. J. ; Hawkey, C. R. ; Greenfield, S. A.</creatorcontrib><description>: In this study, we compare the electrically evoked, somatodendritic release of dopamine (DA) with axonal release of serotonin (5‐HT) in the substantia nigra (SN) and ventral tegmental area (VTA) in vitro by using fast‐scan cyclic voltammetry with carbon‐fibre microelectrodes. Furthermore, we have examined transmitter release in these regions in guinea‐pig compared with rat. Somatodendritic DA was released, as shown previously, in guinea‐pig VTA, SN pars compacta (SNc), and occasionally in SN pars reticulata (SNr). 5‐HT was rarely released, except in SNr, where nonetheless it only contributed to <30% of amine signals. In rat midbrain, somatodendritic DA release was evoked to a similar extent as in guinea‐pig. However, a clear species difference was apparent; i.e., 5‐HT and DA were detected equally in rat SNc, whereas in rat SNr, 5‐HT was the predominant transmitter detected. Nevertheless, electrically evoked extracellular concentrations of 5‐HT in SNc and SNr were, respectively, seven‐ and fourfold less than DA in SNc. 5‐HT release was low in all regions in neonatal rat slices before the maturation of 5‐HT terminals. Hence, axonal 5‐HT transmission in midbrain exhibits both species and site selectivity. Moreover, whereas somatodendritic DA release is conventionally regarded as modest compared with axon terminal release in striatum, somatodendritic DA release can result in significantly greater extracellular levels than a transmitter released from axon terminals in the same locality.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1046/j.1471-4159.1997.69062378.x</identifier><identifier>PMID: 9375669</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Animals, Newborn - metabolism ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Dopamine ; Dopamine - metabolism ; Electric Stimulation ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Male ; Mesencephalon - metabolism ; Rats ; Rats, Wistar ; Serotonin ; Serotonin - metabolism ; Somatodendritic release ; Species difference ; Species Specificity ; Substantia nigra ; Substantia Nigra - metabolism ; Tegmentum Mesencephali - metabolism ; Tissue Distribution ; Ventral tegmental area ; Vertebrates: nervous system and sense organs ; Voltammetry</subject><ispartof>Journal of neurochemistry, 1997-12, Vol.69 (6), p.2378-2386</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4908-34b1ea58bbdf16820e0d99d9b77de445e72c25aee60a58f0c8c23717825af99c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2089563$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9375669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cragg, S. J.</creatorcontrib><creatorcontrib>Hawkey, C. R.</creatorcontrib><creatorcontrib>Greenfield, S. A.</creatorcontrib><title>Comparison of Serotonin and Dopamine Release in Substantia Nigra and Ventral Tegmental Area: Region and Species Differences</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: In this study, we compare the electrically evoked, somatodendritic release of dopamine (DA) with axonal release of serotonin (5‐HT) in the substantia nigra (SN) and ventral tegmental area (VTA) in vitro by using fast‐scan cyclic voltammetry with carbon‐fibre microelectrodes. Furthermore, we have examined transmitter release in these regions in guinea‐pig compared with rat. Somatodendritic DA was released, as shown previously, in guinea‐pig VTA, SN pars compacta (SNc), and occasionally in SN pars reticulata (SNr). 5‐HT was rarely released, except in SNr, where nonetheless it only contributed to <30% of amine signals. In rat midbrain, somatodendritic DA release was evoked to a similar extent as in guinea‐pig. However, a clear species difference was apparent; i.e., 5‐HT and DA were detected equally in rat SNc, whereas in rat SNr, 5‐HT was the predominant transmitter detected. Nevertheless, electrically evoked extracellular concentrations of 5‐HT in SNc and SNr were, respectively, seven‐ and fourfold less than DA in SNc. 5‐HT release was low in all regions in neonatal rat slices before the maturation of 5‐HT terminals. Hence, axonal 5‐HT transmission in midbrain exhibits both species and site selectivity. Moreover, whereas somatodendritic DA release is conventionally regarded as modest compared with axon terminal release in striatum, somatodendritic DA release can result in significantly greater extracellular levels than a transmitter released from axon terminals in the same locality.</description><subject>Animals</subject><subject>Animals, Newborn - metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Dopamine</subject><subject>Dopamine - metabolism</subject><subject>Electric Stimulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Male</subject><subject>Mesencephalon - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Serotonin</subject><subject>Serotonin - metabolism</subject><subject>Somatodendritic release</subject><subject>Species difference</subject><subject>Species Specificity</subject><subject>Substantia nigra</subject><subject>Substantia Nigra - metabolism</subject><subject>Tegmentum Mesencephali - metabolism</subject><subject>Tissue Distribution</subject><subject>Ventral tegmental area</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Voltammetry</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqVkV1v0zAUhi0EGmXwE5AigbhLsB3HH3A1tYwPTUOig1vLcU4qV4md2anYtD-PS7veIq58fN7nPcfyi9AbgiuCGX-_rQgTpGSkURVRSlRcYU5rIau7J2hx0p6iBcaUljVm9Dl6kdIWY8IZJ2foTNWi4Vwt0MMyjJOJLgVfhL5YQwxz8M4XxnfFKkxmdB6KHzCASVDk_nrXptn42Zni2m2i-Qv-Aj9HMxQ3sBlzmauLCOZD9m1cOMxaT2AdpGLl-h4ieAvpJXrWmyHBq-N5jn5efrpZfimvvn_-ury4Ki1TWJY1awmYRrZt1xMuKQbcKdWpVogOGGtAUEsbA8Bxpnpspc2fQYTMzV4pW5-jd4e5Uwy3O0izHl2yMAzGQ9glLRSjQkj5T5DwWgqpcAY_HkAbQ0oRej1FN5p4rwnW-4z0Vu9z0Psc9D4j_ZiRvsvu18c1u3aE7uQ9hpL1t0fdJGuGPhpvXTphFEvV8DpjqwP22w1w_z8v0N-ul4-3-g_RNK9A</recordid><startdate>199712</startdate><enddate>199712</enddate><creator>Cragg, S. J.</creator><creator>Hawkey, C. R.</creator><creator>Greenfield, S. A.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199712</creationdate><title>Comparison of Serotonin and Dopamine Release in Substantia Nigra and Ventral Tegmental Area: Region and Species Differences</title><author>Cragg, S. J. ; Hawkey, C. R. ; Greenfield, S. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4908-34b1ea58bbdf16820e0d99d9b77de445e72c25aee60a58f0c8c23717825af99c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Animals, Newborn - metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Dopamine</topic><topic>Dopamine - metabolism</topic><topic>Electric Stimulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Male</topic><topic>Mesencephalon - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Serotonin</topic><topic>Serotonin - metabolism</topic><topic>Somatodendritic release</topic><topic>Species difference</topic><topic>Species Specificity</topic><topic>Substantia nigra</topic><topic>Substantia Nigra - metabolism</topic><topic>Tegmentum Mesencephali - metabolism</topic><topic>Tissue Distribution</topic><topic>Ventral tegmental area</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Voltammetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cragg, S. J.</creatorcontrib><creatorcontrib>Hawkey, C. R.</creatorcontrib><creatorcontrib>Greenfield, S. A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cragg, S. J.</au><au>Hawkey, C. R.</au><au>Greenfield, S. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Serotonin and Dopamine Release in Substantia Nigra and Ventral Tegmental Area: Region and Species Differences</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1997-12</date><risdate>1997</risdate><volume>69</volume><issue>6</issue><spage>2378</spage><epage>2386</epage><pages>2378-2386</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: In this study, we compare the electrically evoked, somatodendritic release of dopamine (DA) with axonal release of serotonin (5‐HT) in the substantia nigra (SN) and ventral tegmental area (VTA) in vitro by using fast‐scan cyclic voltammetry with carbon‐fibre microelectrodes. Furthermore, we have examined transmitter release in these regions in guinea‐pig compared with rat. Somatodendritic DA was released, as shown previously, in guinea‐pig VTA, SN pars compacta (SNc), and occasionally in SN pars reticulata (SNr). 5‐HT was rarely released, except in SNr, where nonetheless it only contributed to <30% of amine signals. In rat midbrain, somatodendritic DA release was evoked to a similar extent as in guinea‐pig. However, a clear species difference was apparent; i.e., 5‐HT and DA were detected equally in rat SNc, whereas in rat SNr, 5‐HT was the predominant transmitter detected. Nevertheless, electrically evoked extracellular concentrations of 5‐HT in SNc and SNr were, respectively, seven‐ and fourfold less than DA in SNc. 5‐HT release was low in all regions in neonatal rat slices before the maturation of 5‐HT terminals. Hence, axonal 5‐HT transmission in midbrain exhibits both species and site selectivity. Moreover, whereas somatodendritic DA release is conventionally regarded as modest compared with axon terminal release in striatum, somatodendritic DA release can result in significantly greater extracellular levels than a transmitter released from axon terminals in the same locality.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9375669</pmid><doi>10.1046/j.1471-4159.1997.69062378.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 1997-12, Vol.69 (6), p.2378-2386 |
| issn | 0022-3042 1471-4159 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79427788 |
| source | Full-Text Journals in Chemistry (Open access); Wiley-Blackwell Read & Publish Collection |
| subjects | Animals Animals, Newborn - metabolism Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Dopamine Dopamine - metabolism Electric Stimulation Fundamental and applied biological sciences. Psychology Guinea Pigs Male Mesencephalon - metabolism Rats Rats, Wistar Serotonin Serotonin - metabolism Somatodendritic release Species difference Species Specificity Substantia nigra Substantia Nigra - metabolism Tegmentum Mesencephali - metabolism Tissue Distribution Ventral tegmental area Vertebrates: nervous system and sense organs Voltammetry |
| title | Comparison of Serotonin and Dopamine Release in Substantia Nigra and Ventral Tegmental Area: Region and Species Differences |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T21%3A05%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20Serotonin%20and%20Dopamine%20Release%20in%20Substantia%20Nigra%20and%20Ventral%20Tegmental%20Area:%20Region%20and%20Species%20Differences&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Cragg,%20S.%20J.&rft.date=1997-12&rft.volume=69&rft.issue=6&rft.spage=2378&rft.epage=2386&rft.pages=2378-2386&rft.issn=0022-3042&rft.eissn=1471-4159&rft.coden=JONRA9&rft_id=info:doi/10.1046/j.1471-4159.1997.69062378.x&rft_dat=%3Cproquest_cross%3E16387890%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4908-34b1ea58bbdf16820e0d99d9b77de445e72c25aee60a58f0c8c23717825af99c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16387890&rft_id=info:pmid/9375669&rfr_iscdi=true |