The use of human follicular fluid in gamete intra-Fallopian transfer

We undertook a prospective study to compare our gamete intra-Fallopian transfer (GIFT) procedure with or without the use of human follicular fluid (FF) as a constituent for the final spermatozoal suspension and as the tubal transfer medium for both eggs and spermatozoa. We routinely perform an intra...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1989-11, Vol.4 (8), p.931-936
Main Authors: Tucker, M.J., Chan, Y.M., Chan, S.Y.W., Wong, C.J.Y., Mao, K.R., Leong, M.K.H., Leung, C.K.M.
Format: Article
Language:English
Subjects:
Adult
Chorionic Gonadotropin - therapeutic use
Cytological Techniques
egg maturation
Female
Follicle Stimulating Hormone - therapeutic use
follicular fluid
Follicular Fluid - physiology
Gamete Intrafallopian Transfer
GIFT
Humans
Infertility, Female - therapy
Male
Menotropins - therapeutic use
Pregnancy
Semen - cytology
Semen - physiology
spermatozoal capacitation
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Summary:We undertook a prospective study to compare our gamete intra-Fallopian transfer (GIFT) procedure with or without the use of human follicular fluid (FF) as a constituent for the final spermatozoal suspension and as the tubal transfer medium for both eggs and spermatozoa. We routinely perform an intrauterine and intracervical insemination (IUI and ICI) following GIFT, and FF or culture medium was used accordingly as a constituent in this spermatozoal suspension also. When FF was used (26 cycles), clear FF taken from the first egg-bearing follicle was sterilized by micropore filtration, gassed with 5% CO2 in air and warmed to 37°C. This FF was then used to dilute the spermatozoal suspension (50: 50, v/v) for both tubal, uterine and cervical inseminations at least 30 min before transfer, and all transferable eggs were placed into this FF before transfer. Alternatively (30 control cycles), eggs and spermatozoa were prepared and transferred in Earle’s medium supplemented with 10% pooled fetal cord serum. The FF and control patient groups were relatively homogeneous, with no statistically significant differences in ovarian response, oocyte retrieval or transfer or seminal profiles. The outcome of the GIFT procedures using FF or culture medium showed no significant advantage of the use of FF. The clinical pregnancy rate was similar in both groups: 50% (15/30) control; 46.2% (12/26) FF.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a137015