Sequence of DNA 16S/23S spacer region of Leuconostoc œnos ( Œnococcus œni): application to strain differentiation

Leuconostoc œnos is involved in malolactic fermentation occurring during wine-making. An increasing number of wines are being inoculated with malolactic starters to control the process, and the identification and differentiation of selected strains are now indispensable both for quality control of p...

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Bibliographic Details
Published in:Research in microbiology 1997, Vol.148 (1), p.79-86
Main Authors: Le Jeune, C., Lonvaud-Funel, A.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
DNA, Ribosomal
Fermentation
Fundamental and applied biological sciences. Psychology
Genetics
Gram-Positive Cocci - classification
Gram-Positive Cocci - genetics
Leuconoctoc œnos
Leuconostoc - classification
Leuconostoc - genetics
Leuconostoc oenos
Microbiology
PCR
Polymerase Chain Reaction
rDNA
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S
RNA, Transfer, Ala
Sequence Analysis, DNA
Strain differentiation
tRNA
Wine
Wine-making
Œnococcus œni
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Summary:Leuconostoc œnos is involved in malolactic fermentation occurring during wine-making. An increasing number of wines are being inoculated with malolactic starters to control the process, and the identification and differentiation of selected strains are now indispensable both for quality control of production and for commercial purposes. In the present work we evaluated the potential use of the intergenic regions of three L. œnos strains for their differentiation. The three 16S/23S rRIMA intergenic spacers were amplified in vitro by PCR, and sequences were compared. The spacer sequence was highly conserved in all strains. Inside this spacer, a tRNA-Ala gene containing an 18-bp sequence stretch which is conserved in all tRNA genes was discovered. This sequence, together with random primers, was used for characterization of ten L œnos strains by PCR.
ISSN:0923-2508
1769-7123
DOI:10.1016/S0923-2508(97)81903-1