Exercise-induced changes in enzymatic O-methylation of catecholestrogens by erythrocytes of eumenorrheic women

• Published in: Medicine and science in sports and exercise 1997-12, Vol.29 (12), p.1580-1587
• Main Authors: DE CREE, C, VAN KRANENBURG, G, GEURTEN, P, FUJIMURA, Y, KEIZER, H. A
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Amenorrhea - metabolism; Biological and medical sciences; Catechol O-Methyltransferase - blood; Erythrocytes - enzymology; Erythrocytes - metabolism; Estrogens, Catechol - blood; Exercise - physiology; Female; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Humans; Mammalian female genital system; Menstrual Cycle - physiology; Methylation; Space life sciences; Vertebrates: reproduction
• ISSN: 0195-9131; 1530-0315
• DOI: 10.1097/00005768-199712000-00006

The present study was designed to assess the effects of acute exercise and short-term intensive training on catechol-O-methyltransferase (COMT) activity. COMT inactivates catecholamines and converts primary catecholestrogens (CE) into their O-methylated form yielding the 2- (2-MeOE) and 4-methoxyestrogens (4-MeOE). Blood samples were obtained from 15 previously untrained eumenorrheic women (mean +/- SE, VO2max: 43.8 mL x kg-1 x min-1 +/- 0.6) before and after a 5-d intensive training period, at rest and during incremental exercise. COMT activity was determined in the erythrocytes (RBC-COMT) after incubation of blood lysate with primary CE. The formation of both 2- and 4-MeOE was significantly higher (P < 0.05) during the luteal (LPh) than during the follicular phase (FPh). The amount of 2-MeOE formed (FPh: 4.2 +/- 0.2%; LPh: 4.9 +/- 0.2%) was significantly greater than the produced amount of 4-MeOE (FPh: 1.4 +/- 0.1%; LPh: 1.5 +/- 0.1%) (P < 0.05). Both before and after training, incremental exercise did not significantly alter RBC-COMT activity although we observed a trend for RBC-COMT activity increasing proportionally with the exercise intensity. After a brief period of exhaustive training, during rest the formation of 2-MeOE (FPh: +16.7%, LPh: +15.7%) and 4-MeOE (FPh: +28.6%; LPh: +40%) was significantly (P < 0.05) increased. The results of the present study are consistent with earlier findings reporting increased plasma concentrations of O-methylated CE following training. It is concluded that RBC-COMT activity is increased by brief intensive training, but not by acute exercise. We speculate that an increase in COMT-catalyzed O-methylation of CE may indicate that less COMT is available to deactivate norepinephrine.