Insertion of the fusion gene from Newcastle disease virus into a non-essential region in the terminal repeats of fowlpox virus and demonstration of protective immunity induced by the recombinant

1 Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA and 2 Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, U.K. In this paper we report on the identification of nonessential genes in the ter...

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Bibliographic Details
Published in:Journal of general virology 1990-03, Vol.71 (3), p.621-628
Main Authors: Boursnell, M. E. G, Green, P. F, Campbell, J. I. A, Deuter, A, Peters, R. W, Tomley, F. M, Samson, A. C. R, Chambers, P, Emmerson, P. T, Binns, M. M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Cell Line
Chickens
Cloning, Molecular
DNA Transposable Elements
Enzyme-Linked Immunosorbent Assay
fowlpox virus
Fowlpox virus - genetics
Fowlpox virus - immunology
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetic Vectors
Hemagglutination Inhibition Tests
Immunity
Microbiology
Newcastle disease virus - genetics
Newcastle disease virus - immunology
Plasmids
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Virology
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Summary:1 Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA and 2 Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, U.K. In this paper we report on the identification of nonessential genes in the terminal repeats of the avipoxvirus fowlpox virus and the use of these as insertion sites in a vector system. Foreign genes inserted into these sites are shown to be present in two copies in the resultant recombinant virus. To test the potential use of this vector as a live vaccine the fusion gene of Newcastle disease virus has been inserted into a vaccine strain of fowlpox virus and inoculated into chickens. The experiments demonstrate the ability of the recombinant to protect chickens against challenge by a virulent strain of Newcastle disease virus and to elicit the formation of an anti-fusion protein antibody. Present address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K. Received 19 September 1989; accepted 30 November 1989.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-71-3-621