Keratins as Markers that Distinguish Normal and Tumor-Derived Mammary Epithelial Cells

Keratin 5 (K5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. To extend these findings, the full complements of keratins in normal, immortalized, and tumor cells were compared. It is shown here that normal cells...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1990-03, Vol.87 (6), p.2319-2323
Main Authors: Trask, Douglas K., Band, Vimla, Zajchowski, Deborah A., Yaswen, Paul, Suh, Theodore, Sager, Ruth
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
Biomarkers, Tumor - analysis
Blotting, Northern
BODY
Breast
Breast Neoplasms
CARBOXYLIC ACIDS
CARCINOGENESIS
Cell culture techniques
Cell Line
Cell lines
Cells
Complementary DNA
DAYS LIVING RADIOISOTOPES
DNA
DNA - genetics
DNA, Neoplasm - genetics
DRUGS
ELECTROPHORESIS
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Epithelial cells
EPITHELIUM
Epithelium - metabolism
EVEN-ODD NUCLEI
Female
Gels
Gene Library
GLANDS
Humans
ISOTOPES
KERATIN
Keratins
Keratins - analysis
Keratins - biosynthesis
Keratins - genetics
LIGHT NUCLEI
LIPOTROPIC FACTORS
mammary gland
MAMMARY GLANDS
MESSENGER-RNA
METHIONINE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
RNA, Messenger - analysis
SCLEROPROTEINS
SULFUR 35
SULFUR ISOTOPES
TISSUES
Tumor cell line
TUMOR CELLS
Tumor Cells, Cultured - metabolism
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Keratin 5 (K5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. To extend these findings, the full complements of keratins in normal, immortalized, and tumor cells were compared. It is shown here that normal cells produce keratins K5, K6, K7, K14, and K17, whereas tumor cells produce mainly keratins K8, K18, and K19. In immortalized cells, which are preneoplastic or partially transformed, the levels of K5 mRNA and protein are lower than in normal cells, whereas the amount of K18 is increased. Thus, K5 is an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased K5 expression correlates with tumorigenic progression.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.87.6.2319