Epidermal N-acetylation of p-aminobenzoyl glutamic acid: difference in response to ultraviolet B irradiation

Arylamine N-acetyltransferases (EC 2.3.1.5) are conjugating drug-metabolizing enzymes and consist of two major isozymes, NAT1 and NAT2. As they have different substrate specificity and expression of polymorphism, distribution of the isozymes may be detected by investigating acetylation. p-Aminobenzo...

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Bibliographic Details
Published in:Journal of dermatological science 1998, Vol.16 (2), p.99-103
Main Authors: Kawakubo, Yo, Ohkido, Muneo
Format: Article
Language:English
Subjects:
Acetylation
Arylamine N-Acetyltransferase - metabolism
Arylamine N-Acetyltransferase - radiation effects
Cells, Cultured
Epidermis - cytology
Epidermis - enzymology
Glutamates - analysis
Glutamates - metabolism
Glutamates - radiation effects
Humans
Keratinocyte
Kinetics
N-Acetylation
NAT1
NAT2
Time Factors
Ultraviolet Rays
UVB
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Summary:Arylamine N-acetyltransferases (EC 2.3.1.5) are conjugating drug-metabolizing enzymes and consist of two major isozymes, NAT1 and NAT2. As they have different substrate specificity and expression of polymorphism, distribution of the isozymes may be detected by investigating acetylation. p-Aminobenzoyl glutamic acid (pABG), one of the specific substrates for NAT1 in human pro-monocytic cell-line, was metabolized through acetylation by 9000 × g supernatant fraction from human epidermal keratinocytes and the effect of ultraviolet B (UVB) irradiation on the acetylation was also studied. Forty-eight hours after irradiation of UVB (200 J/m 2), the activity was not increased (114 ± 8.3%, n = 3), while increase in N-acetylating capacity for 2-aminofluorene (substrate for NAT1 and NAT2) amounted to 201 ± 16% ( n = 3). These results suggest that there are at least two isozymes for N-acetylationin the human epidermic and NAT2 may be affected by UVB irradiation.
ISSN:0923-1811
1873-569X
DOI:10.1016/S0923-1811(97)00030-3