Genotyping of hepatitis E virus in clinical specimens by restriction endonuclease analysis

The genomic variability of hepatitis E virus (HEV) was examined by restriction endonuclease analysis (REA) of four genomic cDNA copies comprising a 499 bp segment of the putative polymerase gene, a 264 bp segment of the helicase gene, and two, 680 bp and 448 bp, segments of the capsid gene. Analysis...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virological methods 1998, Vol.70 (1), p.71-78
Main Authors: Gouvea, Vera, Hoke, Charles H, Innis, Bruce L
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA Restriction Enzymes - metabolism
DNA, Viral - genetics
DNA, Viral - metabolism
Fundamental and applied biological sciences. Psychology
Genetic Variation
Genotype
Hepatitis E - epidemiology
Hepatitis E - virology
Hepatitis E virus
Hepatitis E virus - classification
Hepatitis E virus - genetics
HEV genotyping
Humans
Microbiology
Multi-site RT-PCR
Polymerase Chain Reaction
Restriction endonuclease analysis
Restriction Mapping
Species Specificity
Techniques used in virology
Transcription, Genetic
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The genomic variability of hepatitis E virus (HEV) was examined by restriction endonuclease analysis (REA) of four genomic cDNA copies comprising a 499 bp segment of the putative polymerase gene, a 264 bp segment of the helicase gene, and two, 680 bp and 448 bp, segments of the capsid gene. Analysis of the deduced restriction sites of all 27 HEV sequences currently available in the GenBank, and digestion of reverse-transcribed and nested PCR amplified segments obtained from six Nepali isolates were used to devise and test a REA genotyping assay. The assay allowed easy discrimination between the Mexico and Asian genotypes, and the classification of the Asian genotypes into three, or perhaps four subgenotypes. In addition, endonucleases identifiers of individual isolate or clusters of isolates were found. This assay permits rapid identification of a large number of HEV isolates directly from clinical specimens for studies on the molecular epidemiology and evolution of HEV.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(97)00172-9