Quantification of Cerebrospinal Fluid Proteins in Children by High-Resolution Agarose Gel Electrophoresis

Physiologic alterations in cerebrospinal fluid proteins occur inter alia with aging. Agarose gel electrophoresis discriminates many cerebrospinal fluid proteins and in addition quantifies concentration alterations. This study aimed to investigate the time course of these alterations in children and...

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Bibliographic Details
Published in:Journal of child neurology 1998-02, Vol.13 (2), p.51-58
Main Authors: Barnard, Karin, Herold, Ralf, Siemes, Hartmut, Siegert, Martin
Format: Article
Language:English
Subjects:
Adolescent
Age Factors
Anatomy
Cerebrospinal Fluid Proteins - analysis
Child
Child, Preschool
Computers
Delivery Systems
Electrophoresis, Agar Gel
Female
Fractions
gamma-Globulins - analysis
Humans
Individualized Instruction
Infant
Infant, Newborn
Laboratory Equipment
Male
Measurement Techniques
Prealbumin - analysis
Reference Values
Serum Albumin - analysis
Spinal Cord - growth & development
Spinal Puncture
Statistical Analysis
Transferrin - analysis
Visual Impairments
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Summary:Physiologic alterations in cerebrospinal fluid proteins occur inter alia with aging. Agarose gel electrophoresis discriminates many cerebrospinal fluid proteins and in addition quantifies concentration alterations. This study aimed to investigate the time course of these alterations in children and to establish normative values for cerebrospinal fluid protein properties. In 202 children without diseases known to alter cerebrospinal fluid, normative protein properties were quantified using nephelometry, ultrafiltration, high-resolution electrophoresis, and Gaussian curve fit densitometry. Total protein and protein concentrations (albumin and γ-globulins) decreased from birth until 7 months age, and, from then on, increased slightly (transthyretin, albumin, and α2-proteins) or strongly (γ-globulins). Protein proportions (transthyretin and transferrin) increased until about 3 years of age and decreased from then on. These normative values for children as quantified by high-resolution agarose gel electrophoresis are presented in a significance-structured percentile table. The time courses of these cerebrospinal fluid properties reflect physiologic alterations of the blood-brain barrier function during childhood. (J Child Neurol 1998;13:51-58).
ISSN:0883-0738
1708-8283
DOI:10.1177/088307389801300201