Real time measurement of stimulated dopamine release in the conscious rat using fast cyclic voltammetry: dopamine release is not observed during intracranial self stimulation

Fast cyclic voltammetry (FCV) was used to measure real time release of electrically stimulated endogenous dopamine in the nucleus accumbens (NAc) of conscious freely moving rats for up to 17 days. The method of electrode construction, implantation, electrical stimulation and recording of changes of...

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Bibliographic Details
Published in:Journal of neuroscience methods 1998-01, Vol.79 (1), p.9-19
Main Authors: Kruk, Z.L, Cheeta, S, Milla, J, Muscat, R, Williams, J.E.G, Willner, P
Format: Article
Language:English
Subjects:
Animals
Behavior, Animal - physiology
Behavioral psychophysiology
Biological and medical sciences
Carbon fibre electrode
Conscious rats
Dopamine - metabolism
Electric Stimulation
Electrical stimulation
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes, Implanted
Endogenous dopamine release
Fast cyclic voltammetry
Fundamental and applied biological sciences. Psychology
Intracranial self stimulation
Male
Methods
Microelectrodes
Nucleus accumbens
Psychology. Psychoanalysis. Psychiatry
Psychology. Psychophysiology
Rats
Rats, Sprague-Dawley
Self Stimulation - physiology
Stereotaxic Techniques
Ventral tegmental area
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Summary:Fast cyclic voltammetry (FCV) was used to measure real time release of electrically stimulated endogenous dopamine in the nucleus accumbens (NAc) of conscious freely moving rats for up to 17 days. The method of electrode construction, implantation, electrical stimulation and recording of changes of extracellular dopamine concentration in the conscious rat are described. Rats trained on a continuous reinforcement schedule to perform intracranial self stimulation (ICSS) were implanted with electrodes for FCV. During ICSS, no faradaic signal was observed at an electrode implanted in the NAc. Decreasing the intensity of the stimulating current abolished ICSS, increasing the stimulating current disrupted ICSS. Operator delivered electrical stimulations using currents greater than those needed for ICSS yielded dopamine signals. It is concluded that during ICSS, sufficient dopamine does not reach the extracellular fluid space to yield a faradaic signal detectable by FCV.
ISSN:0165-0270
1872-678X
DOI:10.1016/S0165-0270(97)00156-8