Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

• Published in: Microbiology (Society for General Microbiology) 1998-01, Vol.144 (1), p.193-199
• Main Authors: Chong, Pei Pei, Podmore, Sylvia M, Kieser, Helen M, Redenbach, Matthias, Turgay, Kursad, Marahiel, Mohamed, Hopwood, David A, Smith, Colin P
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Bacteriology; Base Sequence; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Chromosome Mapping; Chromosomes, Bacterial; DNA Primers; DNA, Bacterial - biosynthesis; DNA, Bacterial - isolation & purification; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; Ionophores - metabolism; Microbiology; Mission oriented research; Molecular Weight; Peptide Synthases - genetics; Peptide Synthases - metabolism; Peptides; Polymerase Chain Reaction; Restriction Mapping; Sequence Alignment; Streptomyces - genetics; Streptomyces - metabolism; Streptomyces coelicolor; Transformation, Bacterial
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-144-1-193

Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK John Innes Centre, Norwich NR4 7UH, UK Department of Genetics, Genome Research Unit, Kaiserslautern University, 67663 Kaiserslautern, Germany FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany ABSTRACT Summary: Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned. Author for correspondence: Colin P. Smith. Tel: + 44 161 200 4183. Fax: [phone]. e-mail: colin.smith@ umist.ac.uk Keywords: Streptomyces coelicolor, antibiotic biosynthesis, lipopeptide, gene disruption, peptide synthetase Present address: Public Health Research Institute, 455 First Avenue, NY 10016, USA.