Impairment of intestinal mucosal antioxidant defense system during Salmonella typhimurium infection

The mucosal pathology of Salmonella typhimurium infection may in part be due to the excessive production of reactive oxygen species (ROS). The influence of S. typhimurium infection on the intestinal mucosal antioxidant defense system was investigated. We injected ligated rat ileal loops with Salmone...

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Bibliographic Details
Published in:Digestive diseases and sciences 1998-03, Vol.43 (3), p.646-651
Main Authors: MEHTA, A, SINGH, S, NIRMAL KUMAR GANGULY
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Biological and medical sciences
Human bacterial diseases
Ileum - metabolism
Infectious diseases
Intestinal Mucosa - metabolism
Intestinal Mucosa - physiopathology
Male
Medical sciences
Rabbits
Rats
Rats, Wistar
Reactive Oxygen Species
Salmonella
Salmonella Infections, Animal - metabolism
Salmonella typhimurium
Tropical medicine
Vitamin A - metabolism
Vitamin E - metabolism
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Summary:The mucosal pathology of Salmonella typhimurium infection may in part be due to the excessive production of reactive oxygen species (ROS). The influence of S. typhimurium infection on the intestinal mucosal antioxidant defense system was investigated. We injected ligated rat ileal loops with Salmonella live culture or toxin. After 18 hr of infection, the animals were killed and enterocytes isolated from the ileal loops. The enterocyte-reduced glutathione (GSH) content and activities of the enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase, glutathione-S-transferase (GST), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PDH) were spectrophotometrically estimated. The vitamin E and A contents were determined by high-performance liquid chromatography (HPLC). In both the Salmonella live culture and toxin-treated groups, the enterocyte GSH and vitamin E contents and activities of the enzymes SOD, GSH-Px, catalase, GR, and G6PDH were significantly decreased as compared to the control group. However there was a significant increase in the enterocyte activity of GST. There was no change in the vitamin A content of the enterocytes. These findings might indicate a decreased endogenous intestinal protection against ROS in S. typhimurium-mediated infection, which could contribute to the pathogenesis of the disease.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1018887813713