Determination of Cytosol c-erbB-2 Protein in Breast Cancer by Sandwich Enzyme Immunoassay

We determined cytosol c-erbB-2 protein levels using a sandwich enzyme immunoassay in benign breast disease and primary and recurrent breast cancer and analyzed the relationship between c-erbB-2 protein levels and clinicopathological factors. Overexpression of c-erbB-2 protein, the cut-off value bein...

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Bibliographic Details
Published in:Japanese journal of clinical oncology 1998-02, Vol.28 (2), p.92-96
Main Authors: Imoto, Shigeru, Ohkura, Hisanao, Sugano, Kokichi, Sasaki, Yasutsuna, Ito, Kuniaki, Igarashi, Tadahiko, Ohtsu, Tomoko, Fujii, Hirofumi, Minami, Hironobu, Hasebe, Takahiro, Mukai, Kiyoshi
Format: Article
Language:English
Subjects:
breast cancer
Breast Diseases - genetics
Breast Diseases - metabolism
Breast Neoplasms - chemistry
Breast Neoplasms - genetics
Breast Neoplasms - pathology
c-erbB-2
Cytosol - chemistry
enzyme immunoassay
Female
Humans
Immunoenzyme Techniques
Neoplasm Staging
Prognosis
Receptor, ErbB-2 - analysis
Receptor, ErbB-2 - metabolism
Receptors, Estrogen - analysis
Receptors, Progesterone - analysis
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Summary:We determined cytosol c-erbB-2 protein levels using a sandwich enzyme immunoassay in benign breast disease and primary and recurrent breast cancer and analyzed the relationship between c-erbB-2 protein levels and clinicopathological factors. Overexpression of c-erbB-2 protein, the cut-off value being set at 18 ng/mg protein, was observed in 26 of the 139 cases if stages I–IIIB breast cancer (18.7%), four of the 12 cases of stage IV breast cancer (33.3%) and seven of the 13 recurrent breast cancer cases (53.8%). The levels of c-erbB-2 protein were significantly different between the stages. Overexpression of c-erbB-2 protein in stages I–IIIB breast cancer was associated with histological grade and serum CEA level, but not with other clinicopathological factors. In addition, there was an inverse correlation in the group of stages I–III plus IV breast cancer between c-erbB-2 protein expression and estrogen receptor status. Overexpression of c-erbB-2 protein can be easily determined in the cytosol fraction together with hormonal receptor by this method. The prognostic importance will be evaluated in ongoing adjuvant trials for operable breast cancer patients.
ISSN:0368-2811
1465-3621
DOI:10.1093/jjco/28.2.92