Characterization of Monomeric and Homodimeric Forms of Osteoclastogenesis Inhibitory Factor

Osteoclastogenesis inhibitory factor (OCIF) is present naturally as two molecular forms, a monomer and a homodimer. The two forms of recombinant human OCIF (rOCIF) produced by Chinese hamster ovary (CHO) cells were purified to homogeneity. Determination of the C-terminal amino-acid sequences of the...

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Published in:Biochemical and biophysical research communications 1998-04, Vol.245 (2), p.382-387
Main Authors: Tomoyasu, Akihiro, Goto, Masaaki, Fujise, Nobuaki, Mochizuki, Shin-ichi, Yasuda, Hisataka, Morinaga, Tomonori, Tsuda, Eisuke, Higashio, Kanji
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Calcium - blood
CHO Cells
Cricetinae
Dimerization
Disulfides - chemistry
Glycoproteins - chemistry
Glycoproteins - pharmacokinetics
Heparin - metabolism
Humans
Molecular Sequence Data
N-Acetylneuraminic Acid - analysis
Osteoprotegerin
Protein Binding - physiology
Receptors, Cytoplasmic and Nuclear
Receptors, Tumor Necrosis Factor
Recombinant Proteins - chemistry
Sequence Analysis
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Summary:Osteoclastogenesis inhibitory factor (OCIF) is present naturally as two molecular forms, a monomer and a homodimer. The two forms of recombinant human OCIF (rOCIF) produced by Chinese hamster ovary (CHO) cells were purified to homogeneity. Determination of the C-terminal amino-acid sequences of the two forms of rOCIF revealed that the monomeric rOCIF lacked several amino acids including Cys379, which is involved in the intermolecular disulfide bond, in its C-terminal region. The two forms of rOCIF were indistinguishable in stability, sialic acid content, and specific activity in inhibition of osteoclastogenesis. In contrast, the homodimeric rOCIF was stronger in heparin-binding ability than the monomeric rOCIF. The homodimeric rOCIF was significantly shorter in initial half-life and smaller in AUC value in rats than the monomeric rOCIF, but exerted more potent biological activity in reducing the calcium concentration in serum of rats than did the monomeric rOCIF.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.8443