Analysis of human papillomavirus type 16 E6-E7 transcription in cervical carcinomas and normal cervical epithelium using the polymerase chain reaction

1 Department of Cancer Studies, Cancer Research Campaign Laboratories and 2 Department of Social Medicine, The Medical School, University of Birmingham, Birmingham B15 2TJ, U.K. Cervical biopsies were collected from Birmingham women having cervical intraepithelial neoplasia or invasive cervical carc...

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Bibliographic Details
Published in:Journal of general virology 1990-07, Vol.71 (7), p.1473-1479
Main Authors: Johnson, M. A, Blomfield, P. I, Bevan, I. S, Woodman, C. B. J, Young, L. S
Format: Article
Language:English
Subjects:
biopsy
carcinoma
cervix
Cervix Uteri - microbiology
DNA, Viral - genetics
DNA, Viral - isolation & purification
epithelium
Epithelium - microbiology
Female
Humans
Keratins - genetics
Oligonucleotide Probes
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
polymerase chain reaction
Polymerase Chain Reaction - methods
promoters
RNA, Viral - genetics
RNA, Viral - isolation & purification
transcription
Transcription, Genetic
Uterine Cervical Neoplasms - microbiology
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Summary:1 Department of Cancer Studies, Cancer Research Campaign Laboratories and 2 Department of Social Medicine, The Medical School, University of Birmingham, Birmingham B15 2TJ, U.K. Cervical biopsies were collected from Birmingham women having cervical intraepithelial neoplasia or invasive cervical carcinoma and normal controls, and examined for the presence of human papillomavirus type 16 (HPV-16) E6–E7 DNA and mRNA using an adaptation of the polymerase chain reaction. HPV-16 E6–E7 sequences were detected in all abnormal biopsies and in 90% of the normal biopsies examined, confirming previous studies describing the high prevalence of cervical HPV-16 infection. While we were unable to identify any qualitative differences in RNA transcripts from the p97 promoter, substantial quantitative differences in HPV-16-specific early region transcripts between normal and cytologically abnormal cervices were observed. These results suggest that although the level of E6–E7 transcription may contribute to the malignant phenotype, additional factors are likely to be important in the development of cervical neoplasia. Received 17 November 1989; accepted 22 February 1990.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-71-7-1473