Effects of chlorhexidine diacetate on ruminal microorganisms

The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 micromolar chlorhexidine diacetate...

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Bibliographic Details
Published in:Current microbiology 1998-06, Vol.36 (6), p.348-352
Main Authors: Attia-Ismail, S.A. (University of Georgia, Athens, GA.), Martin, S.A, Callaway, T.R
Format: Article
Language:English
Subjects:
Acetic acid
Action of physical and chemical agents on bacteria
Animals
Anti-Infective Agents, Local - pharmacology
BACTERIA
Bacteriology
Biological and medical sciences
Chlorhexidine - pharmacology
chlorhexidine diacetate
Feed additives
Fermentation
Fundamental and applied biological sciences. Psychology
Glucose
Lactic Acid - metabolism
Microbiology
MICROORGANISME DU RUMEN
MICROORGANISMOS DEL RUMEN
Microorganisms
Rumen - microbiology
RUMEN MICROORGANISMS
Ruminantia
STREPTOCOCCUS BOVIS
Streptococcus bovis - drug effects
Streptococcus bovis - growth & development
Streptococcus bovis - metabolism
Toluene
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Summary:The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 micromolar chlorhexidine diacetate to glucose medium resulted in a lag in growth by S. bovis JB1, and growth was completely inhibited in the presence of 3.6, 9.0, and 18 micromolar chlorhexidine. When 6.2 micromolar chlorhexidine diacetate was added to glucose medium after 2 h of incubation, glucose utilization and L-lactate production by S. bovis JB1 were reduced. Phosphoenolpyruvate-dependent phosphorylation of 14C-glucose by toluene-treated cells of S. bovis JB1 was inhibited by increasing concentrations (1.8 to 18 micromolar) of chlorhexidine, whereas only the 18 micromolar concentration reduced the membrane potential. Chlorhexidine diacetate was a potent inhibitor of L-lactate and methane production from glucose fermentation by mixed ruminal microorganisms. However, because chlorhexidine also decreased acetate and propionate concentrations and increased ammonia concentrations in mixed-culture incubations, this antimicrobial compound may have limited application as a ruminant feed additive
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900321