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Effects of chlorhexidine diacetate on ruminal microorganisms
The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 micromolar chlorhexidine diacetate...
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| Published in: | Current microbiology 1998-06, Vol.36 (6), p.348-352 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c431t-26a066a5953ce51987d54a9d6cd818b727771b3f2e267e7ebbe803260f0dc1823 |
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| container_end_page | 352 |
| container_issue | 6 |
| container_start_page | 348 |
| container_title | Current microbiology |
| container_volume | 36 |
| creator | Attia-Ismail, S.A. (University of Georgia, Athens, GA.) Martin, S.A Callaway, T.R |
| description | The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 micromolar chlorhexidine diacetate to glucose medium resulted in a lag in growth by S. bovis JB1, and growth was completely inhibited in the presence of 3.6, 9.0, and 18 micromolar chlorhexidine. When 6.2 micromolar chlorhexidine diacetate was added to glucose medium after 2 h of incubation, glucose utilization and L-lactate production by S. bovis JB1 were reduced. Phosphoenolpyruvate-dependent phosphorylation of 14C-glucose by toluene-treated cells of S. bovis JB1 was inhibited by increasing concentrations (1.8 to 18 micromolar) of chlorhexidine, whereas only the 18 micromolar concentration reduced the membrane potential. Chlorhexidine diacetate was a potent inhibitor of L-lactate and methane production from glucose fermentation by mixed ruminal microorganisms. However, because chlorhexidine also decreased acetate and propionate concentrations and increased ammonia concentrations in mixed-culture incubations, this antimicrobial compound may have limited application as a ruminant feed additive |
| doi_str_mv | 10.1007/s002849900321 |
| format | article |
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(University of Georgia, Athens, GA.) ; Martin, S.A ; Callaway, T.R</creator><creatorcontrib>Attia-Ismail, S.A. (University of Georgia, Athens, GA.) ; Martin, S.A ; Callaway, T.R</creatorcontrib><description>The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 micromolar chlorhexidine diacetate to glucose medium resulted in a lag in growth by S. bovis JB1, and growth was completely inhibited in the presence of 3.6, 9.0, and 18 micromolar chlorhexidine. When 6.2 micromolar chlorhexidine diacetate was added to glucose medium after 2 h of incubation, glucose utilization and L-lactate production by S. bovis JB1 were reduced. Phosphoenolpyruvate-dependent phosphorylation of 14C-glucose by toluene-treated cells of S. bovis JB1 was inhibited by increasing concentrations (1.8 to 18 micromolar) of chlorhexidine, whereas only the 18 micromolar concentration reduced the membrane potential. Chlorhexidine diacetate was a potent inhibitor of L-lactate and methane production from glucose fermentation by mixed ruminal microorganisms. However, because chlorhexidine also decreased acetate and propionate concentrations and increased ammonia concentrations in mixed-culture incubations, this antimicrobial compound may have limited application as a ruminant feed additive</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002849900321</identifier><identifier>PMID: 9608746</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Acetic acid ; Action of physical and chemical agents on bacteria ; Animals ; Anti-Infective Agents, Local - pharmacology ; BACTERIA ; Bacteriology ; Biological and medical sciences ; Chlorhexidine - pharmacology ; chlorhexidine diacetate ; Feed additives ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Glucose ; Lactic Acid - metabolism ; Microbiology ; MICROORGANISME DU RUMEN ; MICROORGANISMOS DEL RUMEN ; Microorganisms ; Rumen - microbiology ; RUMEN MICROORGANISMS ; Ruminantia ; STREPTOCOCCUS BOVIS ; Streptococcus bovis - drug effects ; Streptococcus bovis - growth & development ; Streptococcus bovis - metabolism ; Toluene</subject><ispartof>Current microbiology, 1998-06, Vol.36 (6), p.348-352</ispartof><rights>1998 INIST-CNRS</rights><rights>Springer-Verlag New York Inc. 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-26a066a5953ce51987d54a9d6cd818b727771b3f2e267e7ebbe803260f0dc1823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2275547$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9608746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Attia-Ismail, S.A. (University of Georgia, Athens, GA.)</creatorcontrib><creatorcontrib>Martin, S.A</creatorcontrib><creatorcontrib>Callaway, T.R</creatorcontrib><title>Effects of chlorhexidine diacetate on ruminal microorganisms</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 micromolar chlorhexidine diacetate to glucose medium resulted in a lag in growth by S. bovis JB1, and growth was completely inhibited in the presence of 3.6, 9.0, and 18 micromolar chlorhexidine. When 6.2 micromolar chlorhexidine diacetate was added to glucose medium after 2 h of incubation, glucose utilization and L-lactate production by S. bovis JB1 were reduced. Phosphoenolpyruvate-dependent phosphorylation of 14C-glucose by toluene-treated cells of S. bovis JB1 was inhibited by increasing concentrations (1.8 to 18 micromolar) of chlorhexidine, whereas only the 18 micromolar concentration reduced the membrane potential. Chlorhexidine diacetate was a potent inhibitor of L-lactate and methane production from glucose fermentation by mixed ruminal microorganisms. 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Psychology</subject><subject>Glucose</subject><subject>Lactic Acid - metabolism</subject><subject>Microbiology</subject><subject>MICROORGANISME DU RUMEN</subject><subject>MICROORGANISMOS DEL RUMEN</subject><subject>Microorganisms</subject><subject>Rumen - microbiology</subject><subject>RUMEN MICROORGANISMS</subject><subject>Ruminantia</subject><subject>STREPTOCOCCUS BOVIS</subject><subject>Streptococcus bovis - drug effects</subject><subject>Streptococcus bovis - growth & development</subject><subject>Streptococcus bovis - metabolism</subject><subject>Toluene</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqF0c1rFjEQBvAgSn1bPXoUFhTxsjqTr0mgFymtCgUP2vOSzSbtlt1NTXZB_3vz0peKHvSUw_x4wszD2AuEdwhA7wsAN9JaAMHxEduhFLwFa_Ex24GQojVa4VN2XMotAHILeMSOrAZDUu_Y6XmMwa-lSbHxN1PKN-HHOIxLaIbR-bC6NTRpafI2j4ubmnn0OaV87ZaxzOUZexLdVMLzw3vCri7Ov519ai-_fPx89uGy9VLg2nLtQGunrBI-KLSGBiWdHbQfDJqeOBFhLyIPXFOg0PfB1GU0RBg8Gi5O2Jv73Lucvm-hrN08Fh-myS0hbaWjuj0pS_-FSIq4JFnh239DqY0ghRwqffUXvU1brsfYK05Ccm11Ve29qvcpJYfY3eVxdvlnh9Dte-r-6Kn6l4fUrZ_D8KAPxdT568PcFe-mmN3ix_LAOCelJP2OiS517jpXcvUV7f4XQC3FL9bSn94</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Attia-Ismail, S.A. 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(University of Georgia, Athens, GA.) ; Martin, S.A ; Callaway, T.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-26a066a5953ce51987d54a9d6cd818b727771b3f2e267e7ebbe803260f0dc1823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Acetic acid</topic><topic>Action of physical and chemical agents on bacteria</topic><topic>Animals</topic><topic>Anti-Infective Agents, Local - pharmacology</topic><topic>BACTERIA</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chlorhexidine - pharmacology</topic><topic>chlorhexidine diacetate</topic><topic>Feed additives</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose</topic><topic>Lactic Acid - metabolism</topic><topic>Microbiology</topic><topic>MICROORGANISME DU RUMEN</topic><topic>MICROORGANISMOS DEL RUMEN</topic><topic>Microorganisms</topic><topic>Rumen - microbiology</topic><topic>RUMEN MICROORGANISMS</topic><topic>Ruminantia</topic><topic>STREPTOCOCCUS BOVIS</topic><topic>Streptococcus bovis - drug effects</topic><topic>Streptococcus bovis - growth & development</topic><topic>Streptococcus bovis - metabolism</topic><topic>Toluene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Attia-Ismail, S.A. 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(University of Georgia, Athens, GA.)</au><au>Martin, S.A</au><au>Callaway, T.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of chlorhexidine diacetate on ruminal microorganisms</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>36</volume><issue>6</issue><spage>348</spage><epage>352</epage><pages>348-352</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 micromolar chlorhexidine diacetate to glucose medium resulted in a lag in growth by S. bovis JB1, and growth was completely inhibited in the presence of 3.6, 9.0, and 18 micromolar chlorhexidine. When 6.2 micromolar chlorhexidine diacetate was added to glucose medium after 2 h of incubation, glucose utilization and L-lactate production by S. bovis JB1 were reduced. Phosphoenolpyruvate-dependent phosphorylation of 14C-glucose by toluene-treated cells of S. bovis JB1 was inhibited by increasing concentrations (1.8 to 18 micromolar) of chlorhexidine, whereas only the 18 micromolar concentration reduced the membrane potential. Chlorhexidine diacetate was a potent inhibitor of L-lactate and methane production from glucose fermentation by mixed ruminal microorganisms. However, because chlorhexidine also decreased acetate and propionate concentrations and increased ammonia concentrations in mixed-culture incubations, this antimicrobial compound may have limited application as a ruminant feed additive</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>9608746</pmid><doi>10.1007/s002849900321</doi><tpages>5</tpages></addata></record> |
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| ispartof | Current microbiology, 1998-06, Vol.36 (6), p.348-352 |
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| language | eng |
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| subjects | Acetic acid Action of physical and chemical agents on bacteria Animals Anti-Infective Agents, Local - pharmacology BACTERIA Bacteriology Biological and medical sciences Chlorhexidine - pharmacology chlorhexidine diacetate Feed additives Fermentation Fundamental and applied biological sciences. Psychology Glucose Lactic Acid - metabolism Microbiology MICROORGANISME DU RUMEN MICROORGANISMOS DEL RUMEN Microorganisms Rumen - microbiology RUMEN MICROORGANISMS Ruminantia STREPTOCOCCUS BOVIS Streptococcus bovis - drug effects Streptococcus bovis - growth & development Streptococcus bovis - metabolism Toluene |
| title | Effects of chlorhexidine diacetate on ruminal microorganisms |
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