Association of Serum Antibodies to Adherence Lectin with Invasive Amebiasis and Asymptomatic Infection with Pathogenic Entamoeba histolytica

The recognition of the Entamoeba histolytica galactose-inhibitable adherence lectin by antibodies was studied using sera obtained from subjects in South Africa with an amebic liver abscess or asymptomatically pathogenic or nonpathogenic E. histolytica infection and from uninfected regional controls....

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Bibliographic Details
Published in:The Journal of infectious diseases 1990-09, Vol.162 (3), p.768-772
Main Authors: Ravdin, Jonathan I., Jackson, Terry F. H. G., Petri, William A., Murphy, Cheryl F., Ungar, Beth L. P., Gathiram, Vinodh, Skilogiannis, Joanna, Simjee, Ahmed E.
Format: Article
Language:English
Subjects:
Amebiasis
Amebiasis - immunology
Amebic liver abscess
Animals
Antibodies
Antibodies, Protozoan - blood
Antigens
Bacterial diseases
Biological and medical sciences
Blotting, Western
Concise Communications
Entamoeba histolytica
Entamoeba histolytica - immunology
Entamoebiasis - immunology
Enzyme linked immunosorbent assay
Humans
Infections
Infectious diseases
Lectins
Lectins - immunology
Liver abscess
Liver Abscess, Amebic - immunology
Medical sciences
North America
Parasites
South Africa
Virginia
Zymodemes
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Summary:The recognition of the Entamoeba histolytica galactose-inhibitable adherence lectin by antibodies was studied using sera obtained from subjects in South Africa with an amebic liver abscess or asymptomatically pathogenic or nonpathogenic E. histolytica infection and from uninfected regional controls. In addition, sera from healthy American controls or Americans known to be infected with other parasites were studied. Of the 95 sera containing antibodies to total parasite protein, 9596 demonstrated antibodies to the 170-kDa heavy subunit but not to the 35-kDa light lectin subunit. All sera (n = 253) were tested by ELISA for antibodies to lectin: 9996 from liver abscess patients and all 4 fromindividuals asymptomatically infected with pathogenic E. histolytica were positive; all from the 40 healthy American controls and the 29 infected with other parasites were negative (P < .01). The prevalence of serum anti-lectin antibodies was identical (2596) in asymptomatic South Africans with either a nonpathogenic infection or a negative stool culture for E. histolytica. Thus, the presence of serum antibodies to lectin seems to indicate current or prior invasive amebiasis or asymptomatic intestinal infection with pathogenic E. histolytica.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/162.3.768