Microsatellite instability is rare in the clinical course of myelodysplastic syndrome studied with DNA from fresh and paraffin-embedded tissues

Microsatellite instability (MSI) has been reported to occur in various types of malignant neoplasms. We performed a polymerase-chain-reaction-based assay for MSI between the initial and the most recently available ("latest") samples from 23 patients with myelodysplastic syndrome (MDS). Of...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 1998-05, Vol.124 (5), p.231-235
Main Authors: HARADA, S, KOMATSU, H, UEDA, R, SETO, M, NI, H, XU, J.-H, HAYAMI, Y, TSUBOI, K, WAKITA, A, NITTA, M, KATO, T
Format: Article
Language:English
Subjects:
Acute myeloid leukemia
Adult
Aged
Biological and medical sciences
Blast
Deoxyribonucleic acid
Disease Progression
DNA
Female
Gene Expression Regulation, Neoplastic
Hematologic and hematopoietic diseases
Humans
Leukemia
Leukemia - blood
Leukemia - genetics
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical research
Medical sciences
Microsatellite instability
Microsatellite Repeats
Microsatellites
Middle Aged
Myelodysplastic syndrome
Myelodysplastic Syndromes - blood
Myelodysplastic Syndromes - genetics
Paraffin Embedding
Polymerase Chain Reaction
Refractory anemia
Sequence Analysis, DNA
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Summary:Microsatellite instability (MSI) has been reported to occur in various types of malignant neoplasms. We performed a polymerase-chain-reaction-based assay for MSI between the initial and the most recently available ("latest") samples from 23 patients with myelodysplastic syndrome (MDS). Of these patients, 15 were informative at more than three microsatellite loci. Seven patients showed an increase in leukemic cells while 8 patients did not during the interval between the two analyses. Only 1 of the patients, who had refractory anemia with excess blasts, which changed to acute myelogenous leukemia, showed microsatellite alteration at the analysis times. Among all 23 patients, two alterations were detected in the 42 informative paired samples that showed an increase in leukemic cells (4.8%), while none was detected in the 59 paired samples without such an increase. In total, therefore only two alterations were detected among 101 informative paired samples (2%). This indicates that MSI is rare in the clinical course of MDS irrespective of disease status, and is consequently not a critical genetic event for disease progression in most MDS patients.
ISSN:0171-5216
1432-1335
DOI:10.1007/s004320050159