Enantioselective analysis of propafenone in plasma using a polysaccharide-based chiral stationary phase under reversed-phase conditions

• Published in: Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1998-04, Vol.708 (1), p.177-183
• Main Authors: de Gaitani, Cristiane Masetto, Lanchote, Vera Lucia, Bonato, Pierina Sueli
• Format: Article
• Language: English
• Subjects: Analysis; Anti-Arrhythmia Agents - blood; Anti-Arrhythmia Agents - chemistry; Biological and medical sciences; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; General pharmacology; Humans; Isomerism; Medical sciences; Pharmacology. Drug treatments; Propafenone; Propafenone - blood; Propafenone - chemistry; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(97)00652-X

We present a method for the enantioselective analysis of propafenone in human plasma for application in clinical pharmacokinetic studies. Propafenone enantiomers were resolved on a 10-μm Chiralcel OD-R column (250×4.6 mm I.D.) after solid-phase extraction using disposable solid-phase extraction tubes (RP-18). The mobile phase used for the resolution of propafenone enantiomers and the internal standard propranolol was 0.25 M sodium perchlorate (pH 4.0)–acetonitrile (60:40, v/v), at a flow-rate of 0.7 ml/min. The method showed a mean recovery of 99.9% for ( S)-propafenone and 100.5% for ( R)-propafenone, and the coefficients of variation obtained in the precision and accuracy study were below 10%. The proposed method presented quantitation limits of 25 ng/ml and was linear up to a concentration of 5000 ng/ml of each enantiomer.