Stimulatory effect of 660 nm low level laser energy on hypertrophic scar-derived fibroblasts: possible mechanisms for increase in cell counts

Background and Objective Varying effects of red light wavelengths on in vitro cells have been reported. Low level lasers (LLL) are employed to assist wound healing especially for indolent ulcers. On healing, burn wounds may become hypertrophic, resulting in excessive wound contraction, poor cosmesis...

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Bibliographic Details
Published in:Lasers in surgery and medicine 1998, Vol.22 (5), p.294-301
Main Authors: Webb, Cecilia, Dyson, Mary, Lewis, W. H. P.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cell Count
Cell Line
Cicatrix, Hypertrophic - radiotherapy
Diseases of the skin. Cosmetics
Female
Fibroblasts - radiation effects
Humans
in vitro fibroblasts
In Vitro Techniques
Infrared Rays
Laser Therapy
LLLT
Medical sciences
Methylene Blue
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
red light
Statistics, Nonparametric
Citations: Items that this one cites
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Summary:Background and Objective Varying effects of red light wavelengths on in vitro cells have been reported. Low level lasers (LLL) are employed to assist wound healing especially for indolent ulcers. On healing, burn wounds may become hypertrophic, resulting in excessive wound contraction, poor cosmesis, and functional impairment. This study enquired whether 660 nm LLL affected hypertrophic scar‐derived fibroblasts. Study Design/Materials and Methods: The experiments investigated the effect of a 660 nm, 17 mW laser diode at dosages of 2.4 J/cm2 and 4 J/cm2 on cell counts of two human fibroblast cell lines, derived from hypertrophic scar tissue (HSF) and normal dermal (NDF) tissue explants, respectively. The protocol avoided transfer of postirradiated cells. Estimation of fibroblasts utilized the methylene blue bioassay. Results/Conclusion: The post‐660 nm‐irradiated HSFs exhibited very significantly higher cell counts than controls P < 0.01 on days 1–4 (Mann‐Whitney U‐test), and P < 0.01 on days 1–3 for similarly irradiated NDFs. Lasers Surg. Med. 22:294–301, 1998. © 1998 Wiley‐Liss, Inc.
ISSN:0196-8092
1096-9101
DOI:10.1002/(SICI)1096-9101(1998)22:5<294::AID-LSM6>3.0.CO;2-K