Sensitive high-performance liquid chromatographic determination of arbidol, a new antiviral compound, in human plasma

A highly sensitive and selective HPLC method was developed and validated for the determination of arbidol in human plasma. The method involves the liquid–liquid extraction of drug and internal standard from plasma with tert.-butyl methyl ether followed by evaporation and reconstitution in mobile pha...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-06, Vol.810 (1), p.63-69
Main Authors: Metz, Rainer, Muth, Peter, Ferger, Monika, Kukes, Vladimir G, Vergin, Hartmut
Format: Article
Language:English
Subjects:
Antiviral Agents - blood
Arbidol
Calibration
Chromatography, High Pressure Liquid
Drug Stability
Freezing
Humans
Indicators and Reagents
Indoles - blood
Reproducibility of Results
Solutions
Spectrophotometry, Ultraviolet
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Summary:A highly sensitive and selective HPLC method was developed and validated for the determination of arbidol in human plasma. The method involves the liquid–liquid extraction of drug and internal standard from plasma with tert.-butyl methyl ether followed by evaporation and reconstitution in mobile phase. UV detection was done at 315 nm. The limit of quantification for arbidol in plasma was 0.005 μg/ml. Linearity in plasma was proven over the whole calibration range (10.2–0.005 μg/ml). The method was validated according to GLP guidelines and its suitability was demonstrated by analysis of samples from a pharmacokinetic study.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)01006-6