Uptake of lipids by developing oocytes of the hawkmoth Manduca sexta. The possible role of lipoprotein lipase

A plasma membrane preparation of Manduca sexta ovarian follicles was shown to contain lipoprotein lipase activity that hydrolyzes the diacylglycerol moiety of the hemolymph lipoprotein, lipophorin. Kinetic analysis demonstrated that low density lipophorin (LDLp) is a better substrate for the identif...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 1998-05, Vol.28 (5), p.399-408
Main Authors: van Antwerpen, Rik, Salvador, Kirk, Tolman, Ken, Gentry, Cynthia
Format: Article
Language:English
Subjects:
Animals
Biological Transport, Active
Carrier Proteins - metabolism
Cell Membrane - enzymology
Enzyme Inhibitors - metabolism
Enzyme Inhibitors - pharmacology
Enzyme Stability
Female
HDLp
Hemolymph - metabolism
Isoflurophate - metabolism
Isoflurophate - pharmacology
Kinetics
LDLp
Lipid Metabolism
Lipophorin
Lipoprotein Lipase - antagonists & inhibitors
Lipoprotein Lipase - metabolism
Lipoproteins - metabolism
Manduca - growth & development
Manduca - metabolism
Oocytes - growth & development
Oocytes - metabolism
Oogenesis
Ovarian follicle
Protein Binding
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Summary:A plasma membrane preparation of Manduca sexta ovarian follicles was shown to contain lipoprotein lipase activity that hydrolyzes the diacylglycerol moiety of the hemolymph lipoprotein, lipophorin. Kinetic analysis demonstrated that low density lipophorin (LDLp) is a better substrate for the identified lipase activity than high density lipophorin (HDLp). The activity has a pH optimum of 9–9.5 and is partially inhibited by NaCl concentrations higher than 0.5 M. Diisopropylfluorophosphate (DFP) inhibits the membrane bound lipoprotein lipase activity completely and irreversibly. Incubation of follicle plasma membranes with [ 3H]-DFP radiolabels a 100-kDa membrane protein that may represent the lipoprotein lipase. The present results suggest that lipoprotein lipase activity may play an important role in the uptake of lipids by M. sexta oocytes.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(98)00012-5