A Glucose Biosensor Based on Surface-Enhanced Raman Scattering:  Improved Partition Layer, Temporal Stability, Reversibility, and Resistance to Serum Protein Interference

• Published in: Analytical chemistry (Washington) 2004-01, Vol.76 (1), p.78-85
• Main Authors: Yonzon, Chanda Ranjit, Haynes, Christy L, Zhang, Xiaoyu, Walsh, Joseph T, Van Duyne, Richard P
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Blood Proteins - analysis; Carbohydrates; Cattle; Chemistry; Drug Stability; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - analysis; Methods. Procedures. Technologies; Oses; Other biological molecules; Proteins; Spectrometric and optical methods; Spectrum Analysis, Raman - methods; Time Factors; Various methods and equipments
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac035134k

This work updates the recent progress made toward fabricating a real-time, quantitative, and biocompatible glucose sensor based on surface-enhanced Raman scattering (SERS). The sensor design relies on an alkanethiolate tri(ethylene glycol) monolayer that acts as a partition layer, preconcentrating glucose near a SERS-active surface. Chemometric analysis of the captured SERS spectra demonstrates that glucose is quantitatively detected in the physiological concentration range (0−450 mg/dL, 0−25 mM). In fact, 94% of the predicted glucose concentrations fall within regions A and B of the Clarke error grid, making acceptable predictions in a clinically relevant range. The data presented herein also demonstrate that the glucose sensor provides stable SERS spectra for at least 3 days, making the SERS substrate a candidate for implantable sensing. Glucose sensor reversibility and reusability is evaluated as the sensor is alternately exposed to glucose and saline solutions; after each cycle, difference spectra reveal that the partitioning process is largely reversible. Finally, the SERS glucose sensor successfully partitions glucose even when challenged with bovine serum albumin, a serum protein mimic.