Hypothalamic contribution to sleep–wake cycle development

• Published in: Neuroscience 2004, Vol.123 (2), p.575-582
• Main Authors: Karlsson, K.Æ, Kreider, J.C, Blumberg, M.S
• Format: Article
• Language: English
• Subjects: active sleep; Activity Cycles - physiology; Animals; Animals, Newborn; atonia; Biological and medical sciences; Electromyography; Female; Fundamental and applied biological sciences. Psychology; Hypothalamus - physiology; locus coeruleus; Male; Muscle Tonus - physiology; myoclonic twitching; Rats; Rats, Sprague-Dawley; REM sleep; Sleep - physiology; Sleep. Vigilance; ventrolateral preoptic area; Vertebrates: nervous system and sense organs; Wakefulness - physiology
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2003.09.025

Infant mammals cycle rapidly between sleep and wakefulness and only gradually does a more consolidated sleep pattern develop. The neural substrates responsible for this consolidation are unknown. To establish a reliable measure of sleep-wake cyclicity in infant rats, nuchal muscle tone was measured in 2-, 5-, and 8-day-old rats, as were motor behaviors associated with sleep (i.e. myoclonic twitching) and wakefulness (e.g. kicking, stretching). Sleep–wake cycles of 2-day-old rats were characterized by short periods of muscle atonia followed by equally short periods of high tone. In 8-day-olds, sleep periods lengthened significantly and disproportionately in relation to awake periods. Next, locus coeruleus (LC) lesions in 8-day-olds resulted in rapid sleep–wake cycling similar to that exhibited by 2-day-olds; in addition, LC lesions had no effect on the duration of awake periods. Finally, transections caudal, but not rostral, to the anterior hypothalamus also reinstated rapid cycling in 8-day-olds, again without affecting the duration of awake periods. This last finding implicates neural structures within the anterior hypothalamus (e.g. ventrolateral preoptic area) in the modulation of sleep–wake cyclicity. The temporal coherence of atonia and myoclonic twitching was not disrupted by any of the manipulations. These results suggest the presence of a bistable mesopontine circuit governing rapid sleep–wake cycling that does not include the LC and that comes increasingly under hypothalamic control during the first postnatal week. This circuit may represent a basic building block with which other sleep components become integrated during ontogeny.