Temperature regulates bacterial protein production: possible role in rosacea

• Published in: Journal of the American Academy of Dermatology 2004-02, Vol.50 (2), p.266-272
• Main Authors: Dahl, Mark V, Ross, Amy J, Schlievert, Patrick M
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - secretion; Biological and medical sciences; Case-Control Studies; Dermatology; Hemolysin Proteins - analysis; Humans; Medical sciences; Rosacea - physiopathology; Skin involvement in other diseases. Miscellaneous. General aspects; Skin Temperature; Staphylococcus epidermidis - physiology
• ISSN: 0190-9622; 1097-6787
• DOI: 10.1016/j.jaad.2003.05.005

Facial skin temperature is higher for patients with rosacea. Papules and pustules might arise because bacteria behave differently at these warmer temperatures. We sought to: (1) compare bacteria from facial skin of patients with rosacea with that of control subjects; and (2) grow these bacteria at 30°C and 37°C to compare growth curves and secreted proteins. Bacteria isolated from pustules/skin surfaces of patients with rosacea and skin surfaces of control subjects were identified and cultured at 37°C and 30°C. Secreted proteins were separated by electrophoresis. We found that Staphylococcus epidermidis isolated from patients with rosacea was consistently β-hemolytic, whereas that from control subjects were nonhemolytic. Bacteria from patients with rosacea grew at the same rate and to the same stationary phase whether cultured at 37°C or 30°C. Isolates from patients with rosacea secreted more proteins, and generally more of each protein at 37°C compared with 30°C. In conclusion, bacteria isolated from patients with rosacea secrete different proteins and different amounts of protein at different temperatures.