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Detection of Micrometastatic Disease and Monitoring of Perioperative Tumor Cell Dissemination in Primary Operable Breast Cancer Patients Using Real-Time Quantitative Reverse Transcription-PCR
Purpose: We previously found a statistically significant number of cytokeratin 19 (CK19)+ cells in peripheral blood (PB) of stage IV breast cancer (BC) patients compared with those of healthy volunteers, using a quantitative real-time reverse transcription-PCR. We aimed to apply the technique on bon...
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Published in: | Clinical cancer research 2004-01, Vol.10 (1), p.196-201 |
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Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Purpose: We previously found a statistically significant number of cytokeratin 19 (CK19)+ cells in peripheral blood (PB) of stage
IV breast cancer (BC) patients compared with those of healthy volunteers, using a quantitative real-time reverse transcription-PCR.
We aimed to apply the technique on bone marrow (BM) of primary operable BC patients. Pre- and postoperative PB samples of
these patients were further analyzed to investigate possible shedding of CK19+ cells during the operation.
Experimental Design: In 54 primary operable BC patients, we analyzed 50 BM samples taken preoperatively and 297 PB samples. PB samples were collected
before surgery; immediately after surgery; on the first, second, and fifth day postoperatively; and one month postoperatively.
Results: In BM of controls and BC patients, we detected a median of 28 and 568 CK19+ cells/5 Ă— 10 6 leukocytes, respectively ( P < 0.001). In preoperative blood (B-1) samples, we measured a median of 109 CK19+ cells. Using the upper limit of 95% confidence
interval of controls as cutoff, 74% and 52% of BM and (B-1), respectively were considered CK19+. There was no significant
correlation between CK19+ cells in BM and (B-1) and classical prognostic factors. We found no significant difference between
blood samples at different time points with respect to the average CK19+ cells.
Conclusions: In primary BC patients, we detected high numbers of CK19+ cells in BM and PB (B-1) samples compared with controls. However,
no significant correlation between the presence of CK19+ cells in BM and PB and classical prognostic factors was found. We
detected no statistically significant influence of surgical manipulation on CK19+ cells. |
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ISSN: | 1078-0432 1557-3265 |
DOI: | 10.1158/1078-0432.CCR-0515-2 |