Morphological changes during dendritic cell maturation correlate with cofilin activation and translocation to the cell membrane

Upon activation, tissue residing immature dendritic cells (DC) start to migrate towards the draining lymph node and mature into efficient antigen‐presenting cells. During maturation DC loose their capacity to endocytose antigens, change their surface expression of adhesion molecules, chemokine recep...

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Bibliographic Details
Published in:European journal of immunology 2004-01, Vol.34 (1), p.156-164
Main Authors: Verdijk, Pauline, van Veelen, Peter A., de Ru, Arnoud H., Hensbergen, Paul J., Mizuno, Kensaku, Koerten, Henk K., Koning, Frits, Tensen, Cornelis P., Mommaas, A. Mieke
Format: Article
Language:English
Subjects:
Actin Depolymerizing Factors
Actins - metabolism
Cell Differentiation - physiology
Cell Membrane - metabolism
Dendritic cells
Dendritic Cells - immunology
Dendritic Cells - physiology
Dendritic Cells - ultrastructure
Fluorescent Antibody Technique
Human
Humans
Lipopolysaccharide
Lipopolysaccharides - metabolism
Microfilament Proteins - immunology
Microfilament Proteins - metabolism
Microscopy, Electron, Scanning
Protein phosphorylation
Protein Transport - physiology
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Summary:Upon activation, tissue residing immature dendritic cells (DC) start to migrate towards the draining lymph node and mature into efficient antigen‐presenting cells. During maturation DC loose their capacity to endocytose antigens, change their surface expression of adhesion molecules, chemokine receptors, and costimulatory molecules, and change morphology. We employed 2D‐PAGE and mass spectrometry to identify additional differentially expressed proteins in immature and mature DC. Human monocyte‐derived DC were matured with LPS and protein expression profiles were compared before andafter maturation. One of the proteins differentially expressed between immature and mature DC was identified as the actin‐binding protein cofilin. We show here that cofilin is dephosphorylated in response to several maturation stimuli (i.e. CD40 ligand, LPS or a combination of TNF‐α and prostaglandin E2). Moreover, dephosphorylated cofilin translocated towards the plasmamembrane during maturation. Importantly, this correlated with an increase in filamentous actin and the appearance of veils, suggesting a role for cofilin in cytoskeletal rearrangements during maturation.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.200324241