Abnormalities of sperm chromosome condensation in the cytoplasm of immature human oocytes

This study analysed data from 27 couples in an IVF-ET programme. The maternal age range was 28–43 years. Statistical analyses on 182 oocytes showed no maternal age effect on the number of oocytes, their stage of maturation or their fertilization rate. There was also no effect of age of either partne...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1990-10, Vol.5 (7), p.842-846
Main Authors: Zenzes, Maria Teresa, de Geyter, C., Bordt, J., Schneider, H.P.G., Nieschlag, E.
Format: Article
Language:English
Subjects:
Adult
Aging
Biological and medical sciences
Birth control
chromosome
Chromosome Aberrations
Chromosome Disorders
Chromosomes - physiology
condensation
Cytoplasm - ultrastructure
embryos
Female
Fertilization in Vitro
Gynecology. Andrology. Obstetrics
Humans
IVF
Male
Medical sciences
oocytes
Oocytes - physiology
Oocytes - ultrastructure
Sperm-Ovum Interactions
Spermatozoa - ultrastructure
Sterility. Assisted procreation
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Summary:This study analysed data from 27 couples in an IVF-ET programme. The maternal age range was 28–43 years. Statistical analyses on 182 oocytes showed no maternal age effect on the number of oocytes, their stage of maturation or their fertilization rate. There was also no effect of age of either partner or of seminal parameters on the fertilization rate. In contrast, occurrence of diploid oocytes was confined to three of the older women. The proportion of failures of fertilization was significantly higher in immature oocytes. These failures, which included 18 uncleaved, multipronuclear or fragmented zygotes, were related to disturbances of oocyte maturation. Four (out of five) oocytes re-inseminated with fresh semen produced polyspermy. One zygote showed marked asynchrony in the development of the two pronuclei. In eight zygotes the paternal complements had an allocyclic pattern of chromosome condensation between and within chromosomes or chromosome regions. In two other zygotes the paternal complement showed one chromosome prematurely condensed. This single-chromatid chromosome would be lost in the following cleavage division, suggesting that aneuploidy due to ‘anaphase lag’ is not a rare event during embryo cleavage.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a137195