Chemical modifications of Pokeweed antiviral protein: effects upon ribosome inactivation, antiviral activity and cytotoxicity

Pokeweed antiviral protein (PAP) is a protein known to inactivate eukaryotic ribosomes by an unknown enzymatic action and inhibit the production of mammalian viruses in tissue culture. This protein was subjected to a variety of chemical modifications to determine their effects upon ribosomal inactiv...

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Bibliographic Details
Published in:FEBS letters 1982-11, Vol.148 (1), p.127-130
Main Authors: Irvin, James D., Aron, Gary M.
Format: Article
Language:English
Subjects:
Animals
antiviral activity
Arginine - metabolism
Cells, Cultured
Cercopithecus aethiops
chemical modification
Chemical modifications
Cytotoxicity
Herpes simplex virus
N-Glycosyl Hydrolases
Phenylalanine - metabolism
Phytolacca americana
Plant Proteins
Pokeweed antiviral protein
Protein Biosynthesis
Protein synthesis
proteins
Ribosome
Ribosome Inactivating Proteins, Type 1
ribosomes
Ribosomes - drug effects
Simplexvirus - drug effects
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Summary:Pokeweed antiviral protein (PAP) is a protein known to inactivate eukaryotic ribosomes by an unknown enzymatic action and inhibit the production of mammalian viruses in tissue culture. This protein was subjected to a variety of chemical modifications to determine their effects upon ribosomal inactivation, antiviral action, and cytotoxicity. It was found that modifications of a number of different amino acid residues had similar effects upon all 3 activities. Also the inactivation of PAP with diethylpyrocarbonate was not due to its reaction with a histidine residue but to a modification of an unidentified amino acid residue.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(82)81257-X