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Chemical modifications of Pokeweed antiviral protein: effects upon ribosome inactivation, antiviral activity and cytotoxicity

Pokeweed antiviral protein (PAP) is a protein known to inactivate eukaryotic ribosomes by an unknown enzymatic action and inhibit the production of mammalian viruses in tissue culture. This protein was subjected to a variety of chemical modifications to determine their effects upon ribosomal inactiv...

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Published in:	FEBS letters 1982-11, Vol.148 (1), p.127-130
Main Authors:	Irvin, James D., Aron, Gary M.
Format:	Article
Language:	English
Subjects:	Animals antiviral activity Arginine - metabolism Cells, Cultured Cercopithecus aethiops chemical modification Chemical modifications Cytotoxicity Herpes simplex virus N-Glycosyl Hydrolases Phenylalanine - metabolism Phytolacca americana Plant Proteins Pokeweed antiviral protein Protein Biosynthesis Protein synthesis proteins Ribosome Ribosome Inactivating Proteins, Type 1 ribosomes Ribosomes - drug effects Simplexvirus - drug effects
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description	Pokeweed antiviral protein (PAP) is a protein known to inactivate eukaryotic ribosomes by an unknown enzymatic action and inhibit the production of mammalian viruses in tissue culture. This protein was subjected to a variety of chemical modifications to determine their effects upon ribosomal inactivation, antiviral action, and cytotoxicity. It was found that modifications of a number of different amino acid residues had similar effects upon all 3 activities. Also the inactivation of PAP with diethylpyrocarbonate was not due to its reaction with a histidine residue but to a modification of an unidentified amino acid residue.
doi_str_mv	10.1016/0014-5793(82)81257-X
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source	ScienceDirect Journals
subjects	Animals antiviral activity Arginine - metabolism Cells, Cultured Cercopithecus aethiops chemical modification Chemical modifications Cytotoxicity Herpes simplex virus N-Glycosyl Hydrolases Phenylalanine - metabolism Phytolacca americana Plant Proteins Pokeweed antiviral protein Protein Biosynthesis Protein synthesis proteins Ribosome Ribosome Inactivating Proteins, Type 1 ribosomes Ribosomes - drug effects Simplexvirus - drug effects
title	Chemical modifications of Pokeweed antiviral protein: effects upon ribosome inactivation, antiviral activity and cytotoxicity
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