Response of erythrocytes to heat in the presence of D2O, glycerol, and anisotonic saline

Mammalian erythrocytes that lack cytoplasmic organelles and a nucleus are a useful model for studying the effect of heat on the cell membrane and cytoskeleton. The effect of heat on the membrane bilayer and cytoskeleton of erythrocytes is remarkably similar to that observed in nucleated cells. Some...

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Bibliographic Details
Published in:Radiation research 1991, Vol.125 (1), p.20-27
Main Authors: Ho, K C, Lin, P S
Format: Article
Language:English
Subjects:
Acclimatization - drug effects
Acclimatization - physiology
Animals
Biological and medical sciences
Cell Membrane
Chickens
Cytoskeleton
Deuterium - pharmacology
Erythrocytes - cytology
Erythrocytes - drug effects
Glycerol - pharmacology
Hot Temperature - adverse effects
Humans
In Vitro Techniques
Medical sciences
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Sodium Chloride - pharmacology
Space life sciences
Species Specificity
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Summary:Mammalian erythrocytes that lack cytoplasmic organelles and a nucleus are a useful model for studying the effect of heat on the cell membrane and cytoskeleton. The effect of heat on the membrane bilayer and cytoskeleton of erythrocytes is remarkably similar to that observed in nucleated cells. Some concentrations of D2O and glycerol can effectively protect erythrocytes from heat-induced damage to the membrane and cytoskeleton. These results are similar to observations in nucleated cells. Heating erythrocytes in some concentrations of anisotonic NaCl solutions reduced damage, an observation that does not apply to enhanced killing of nucleated cells. This difference implies that some components of the cytoplasm or nucleus, or both, may contribute to the enhancement of cytotoxicity of nucleated cells when they are heated in the anisotonic NaCl solution. Incremental heating, dividing a heat treatment into two fractions, and preheating of erythrocytes all modify the effect of heat on erythrocytes slightly, but the results suggest little, if any, development of thermotolerance. The response of chicken erythrocytes is similar to that of mammalian erythrocytes, although higher temperatures are required to produce a heat effect in chicken erythrocytes. These observations suggest that the characteristic differences in heat sensitivity in nucleated and enucleated cells involve components other than the cell membrane.
ISSN:0033-7587
1938-5404
DOI:10.2307/3577977