A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants

• Published in: Plant molecular biology 1990-12, Vol.15 (6), p.921-932
• Main Authors: FEJES, E, PAY, A, KANEVSKY, I, SZELL, M, ADAM, E, KAY, S, NAGY, F
• Format: Article
• Language: English
• Subjects: Amino Acid Oxidoreductases - genetics; Bacterial Proteins - genetics; Base Sequence; Biological and medical sciences; biological rhythms; Circadian Rhythm; Enhancer Elements, Genetic; expresion genica; expression des genes; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation; Genes, Plant; Genes, Synthetic; Genes, Viral; Glucuronidase - biosynthesis; Glucuronidase - genetics; Light-Harvesting Protein Complexes; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mosaic Viruses - genetics; nicotiana; Photosynthetic Reaction Center Complex Proteins - genetics; plantas transgenicas; plante transgenique; Promoter Regions, Genetic; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - genetics; Regulatory Sequences, Nucleic Acid; Rhizobium - genetics; ritmos biologicos; rythme biologique; Terminator Regions, Genetic; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; transgenic plants; triticum; Triticum - genetics; Triticum aestivum
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00039431

We previously reported that the expression of the wheat Cab-1 gene is regulated by an endogenous circadian rhythm and by the photoreceptor phytochrome both in wheat and in transgenic tobacco plants. To define regulatory elements necessary for the circadian rhythm-regulated Cab-1 gene expression, we now analysed the fluctuation of steady-state mRNA levels in a series of 5' deletion mutants in transgenic tobacco plants. We found that the expression of a deletion mutant containing 211 bp upstream sequence still exhibited circadian rhythm. Furthermore we show that an enhancer-like sequence of the Cab-1 promoter (from -357 to -90) can endow a chimaeric gene consisting of a truncated 35S promoter (from -90 to +8) and the bacterial beta-glucuronidase (GUS) gene with circadian clock-regulated gene expression. Finally we demonstrate by nuclear run-off experiments that the transcription rates of the Cab genes in wheat oscillate in a rhythmic manner, with a periodicity of approximately 24 hours. Consistent with our previous findings these results (i) indicate that the expression of the wheat Cab-1 gene is regulated mainly at the transcription level and (ii) identify a short promoter region between -211 and -90 that is responsible for the circadian clock-regulated gene expression.