Biochemical characterization of a calcium ion stimulated-ATPase from goat spermatozoa

• Published in: Molecular and cellular biochemistry 1991-05, Vol.103 (2), p.121-130
• Main Authors: Sikdar, R. (Bose Institute, Calcutta, India), Ganguly, U, Pal, P, Mazumder, B, Sen, P.C
• Format: Article
• Language: English
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Adenosine Triphosphate - metabolism; Animals; Ca(2+) Mg(2+)-ATPase - metabolism; CALCIO; CALCIUM; Calcium-Transporting ATPases - chemistry; Calcium-Transporting ATPases - isolation & purification; Calcium-Transporting ATPases - metabolism; CAPRIN; CAPRINOS; Chemical Fractionation; Detergents - pharmacology; Dithionitrobenzoic Acid - pharmacology; Electrophoresis, Polyacrylamide Gel; ESPERMATOZOO; Exact sciences and technology; Goats; HIDROLASAS; HYDROLASE; Immunoenzyme Techniques; INHIBIDORES DE ENZIMAS; INHIBITEUR D'ENZYME; MAGNESIO; MAGNESIUM; Male; Mathematical analysis; Mathematics; Microsomes - enzymology; Molecular Weight; Potential theory; PURIFICACION; PURIFICATION; Sciences and techniques of general use; spermatozoa; Spermatozoa - enzymology; SPERMATOZOIDE; Substrate Specificity; TEMPERATURA; TEMPERATURE; Trifluoperazine - pharmacology; Trinitrobenzenesulfonic Acid - pharmacology; Vanadates - pharmacology
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/BF00227478

The goat spermatozoa membranes isolated after treatment with octa (ethylene glycol) mono n-dodecyl ether (C12E8) followed by discontinuous sucrose density gradient centrifugation have been found to contain an ATPase that is stimulated by externally added Ca2+ only. The membrane fraction has also found to contain Mg2+ -dependent Ca2+ -ATPase activity, however the former activity is about 2 fold higher than the latter. The molecular weight of the enzyme is found to be about 97,000 on SDS-polyacrylamide gel. The optimum concentration of Ca2+ required for maximum activity is 3mM for both Mg2+ -dependent and Mg2+ -independent Ca2+-ATPase. Histidine and imidazole buffers are found to be the most suitable for dependent and independent enzyme activities respectively. ATP with an optimum concentration of 4mM is observed to be the best substrate than any other nucleotides. The inhibitors like trifluoperazine and vanadate and group specific probes e.g. DTNB and TNBS inhibit these two enzymes but at different rates. Ca2+ -uptake study shows that the uptake in the presence of Ca2+ and ATP is higher than in the presence of Mg2+, Ca2+ -uptake study shows that the uptake in the presence of Ca2+ and ATP is higher than in the presence of Mg2+, Ca2+ and ATP. The findings leads us to believe that the Mg2+ -independent Ca2+-ATPase has some role in Ca2+ transport like Mg2+ -dependent enzyme