Calcium-binding sites in the inner ear after pure-tone stimulation

Five guinea pigs were exposed to an interrupted 90 dB SPL pure tone of 3.2 kHz for a total application time of 5 min. Following sound application all animals were decapitated and the cochleae were removed. After that, calcium-binding sites were located by the potassium pyroantimonate precipitation m...

Full description

Saved in:
Bibliographic Details
Published in:European archives of oto-rhino-laryngology 1991-01, Vol.248 (4), p.242-245
Main Authors: Maurer, J, Mann, W, Heinrich, U R
Format: Article
Language:English
Subjects:
Acoustic Stimulation
Animals
Binding Sites
Calcium - metabolism
Ear, Inner - metabolism
Guinea Pigs
Sound
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Five guinea pigs were exposed to an interrupted 90 dB SPL pure tone of 3.2 kHz for a total application time of 5 min. Following sound application all animals were decapitated and the cochleae were removed. After that, calcium-binding sites were located by the potassium pyroantimonate precipitation method. Another three animals served as control animals and did not receive the sound treatment. Findings confirmed previous studies showing the spatial arrangements of precipitate rich regions in the inner ear's two acellular structures (the basilar membrane and tectorial membrane) and the two cellular structures (the inner hair cells and Huschke's teeth). By using semiquantitative evaluation we found a relative diminution of precipitable calcium in inner hair cells and in Huschke's teeth of sound-exposed animals when compared to untreated animals.
ISSN:0937-4477
1434-4726
DOI:10.1007/BF00173664