Design, expression and characterization of recombinant hybrid peptide Attacin-Thanatin in Escherichia coli

• Published in: Molecular biology reports 2010-10, Vol.37 (7), p.3495-3501
• Main Authors: Wang, Li Na, Yu, Bing, Han, Guo Quan, He, Jun, Chen, Dai Wen
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Antimicrobial Cationic Peptides - genetics; Antimicrobial Cationic Peptides - isolation & purification; Antimicrobial Cationic Peptides - metabolism; Antimicrobial Cationic Peptides - pharmacology; Biomedical and Life Sciences; Cellular biology; E coli; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - metabolism; Gene expression; Histology; Life Sciences; Microbial Sensitivity Tests; Molecular biology; Morphology; Peptides; Plasmids - genetics; Protein Engineering - methods; Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - isolation & purification; Recombinant Fusion Proteins - metabolism; Recombinant Fusion Proteins - pharmacology; Salmonella choleraesuis; Staphylococcus aureus
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-009-9942-3

Antimicrobial peptides will be attractive and potential candidates as peptide drugs because of their efficient action against microbes and low toxicity to mammal cells. To improve their antibacterial activity, some modifications needs to be made. In this research, the hybrid peptide gene Attacin-Thanatin with 642 bp in length with preferred codons of E. coli was generated using the technology of Gene splicing by overlap extension. The gene was inserted in-frame into E. coli expression plasmid pET-32a (+) and induced to express in E. coli Rosetta . The recombinant protein was partial purified and its biological activity was determined. Analysis of the E. coli Rosetta induced with IPTG revealed that the molecular weight of fusion protein was approximately 41.8 kDa, which perfectly matched the mass calculated from the amino acid sequence. Biological activity detection showed that this peptide effectively inhibited the growth of the test bacteria including E. coli DH5α, E. coli BL21 (DE3), Salmonella choleraesuis and Staphylococcus aureus . Among these bacteria, the Gram-negative E. coli was the most sensitive. Furthermore, there was minor hemolysis activity for porcine red blood cells. So, the results indicated that the hybrid peptide Attacin-Thanatin could be served as a promising candidate for the chemical antibiotics.