Gastritis Induced by Helicobacter pylori Infection in Experimental Rats

• Published in: Digestive diseases and sciences 2010-10, Vol.55 (10), p.2770-2777
• Main Authors: Elseweidy, Mohamed M, Taha, Mona M, Younis, Nahla N, Ibrahim, Khadiga S, Hamouda, Hamdi A, Eldosouky, Mohamed A, Soliman, Hala
• Format: Article
• Language: English
• Subjects: Animals; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Biochemistry; Biological and medical sciences; Biomarkers - blood; Biopsy; Cancer; Chemotherapy; Dinoprostone - metabolism; Disease Models, Animal; DNA Fragmentation; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Gastric Mucosa - metabolism; Gastric Mucosa - microbiology; Gastric Mucosa - pathology; Gastrin; Gastrins - metabolism; Gastritis; Gastritis - metabolism; Gastritis - microbiology; Gastritis - pathology; Gastroenterology; Health aspects; Helicobacter infections; Helicobacter Infections - metabolism; Helicobacter Infections - pathology; Helicobacter pylori; Helicobacter pylori - isolation & purification; Hepatology; Human bacterial diseases; Humans; Infection; Infectious diseases; Interleukin-6 - metabolism; Interleukins; Male; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Nitric oxide; Nitric Oxide Synthase Type II - metabolism; Oncology; Original Article; Pepsinogen A - metabolism; Peroxidase - metabolism; Pyloric Antrum - metabolism; Pyloric Antrum - microbiology; Pyloric Antrum - pathology; Rats; Rats, Wistar; Transplant Surgery; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-009-1103-y

Background Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. Aims Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. Methods Gastritis was induced in 20 albino Wistar rats by H. pylori isolated from antral biopsy taken from a 49-year-old male patient endoscopically diagnosed as having H. pylori infection. Another ten rats were used as controls. Serum gastrin, pepsinogen I activity, interleukin-6 (IL-6) and gastric mucosal myeloperoxidase (MPO) activity and prostaglandin E₂ (PGE₂) were measured. Immunostaining for inducible nitric oxide synthase (iNOS), nitrotyrosine and DNA fragmentation were used to further evaluate H. pylori-induced gastritis. Results Serum gastrin, IL-6, mucosal MPO activity, and PGE₂ demonstrated significant increases joined with a decreased serum pepsinogen I activity (P < 0.001). Immunohistochemistry demonstrated positive reaction for iNOS, nitrotyrosine and DNA fragmentation. Conclusions Helicobacter pylori-induced gastritis models demonstrated massive oxidative stress and pronounced injury in mucosal tissue. Since our model in rats reflected the clinical picture of H. pylori infection, it can be considered as a consistent model to study chemotherapeutic intervention for this type of gastritis.