Synchrotron excitation of DNA fluorescence: Decay time evidence for excimer emission at room temperature

The first lifetime measurements of DNA fluorescence are reported. Natural and synthetic DNA have been excited by 1.76 ns pulses of synchrotron ultraviolet radiation (270 nm) and the time profile of the fluorescence has been measured by synchronous single-photon counting. A post-pulse exponentially d...

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Bibliographic Details
Published in:Biophysical chemistry 1983-01, Vol.18 (1), p.61-65
Main Authors: Ballini, J.P., Vigny, P., Daniels, M.
Format: Article
Language:English
Subjects:
DNA fluorescence
Excimer fluorescence
Fluorescence lifetime
Poly(dA-T) fluorescence
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Summary:The first lifetime measurements of DNA fluorescence are reported. Natural and synthetic DNA have been excited by 1.76 ns pulses of synchrotron ultraviolet radiation (270 nm) and the time profile of the fluorescence has been measured by synchronous single-photon counting. A post-pulse exponentially decaying emission has been observed with a lifetime of 2.9 ± 0.4 ns for calf thymus DNA and 3.0 ± 0.3 ns for poly(dA-T); this is most likely an excimer fluorescence.
ISSN:0301-4622
1873-4200
DOI:10.1016/0301-4622(83)80027-1