Effects of monocular deprivation on the distribution of cell types in the LGNd: a sampling study with fine-tipped micropipettes

• Published in: Experimental brain research 1984, Vol.53 (2), p.451-461
• Main Authors: Friedlander, M J, Stanford, L R
• Format: Article
• Language: English
• Subjects: Animals; Axons - physiology; Cats; Functional Laterality; Geniculate Bodies - physiology; Neurons - physiology; Ocular Physiological Phenomena; Sensory Deprivation; Vision, Ocular; Visual Perception
• ISSN: 0014-4819; 1432-1106
• DOI: 10.1007/BF00238175

Six cats were reared with monocular eyelid suture from 1 week of age. Two control animals were reared in the same colony. The sutured eye of the monocularly deprived (MD) cats was opened at 8-9 months of age. The effect of monocular deprivation on the distribution of functional classes of neurons in the dorsal lateral geniculate nucleus (LGNd) was evaluated by extracellularly recording the activity of single neurons in the A-laminae of the LGNd ipsilateral to the deprived eye. A similar sampling procedure was used in the LGNd of the two control animals. Recordings were made with extremely fine-tipped micropipettes (impedances = 50-80 M omega at 200 Hz when filled with 3M NaCl; tip diameters less than 0.3 micron when measured with scanning electron-microscopical methods). Micropipettes with these tip sizes were shown previously (Friedlander et al. 1981) to display no sampling bias on the basis of soma size in the LGNd of normal cats. Only data from complete penetrations through the non-deprived and deprived laminae were used in our analysis. Each animal's non-deprived lamina A also provided control data. In addition, we recorded from geniculocortical axons in the optic radiations above the LGNd (ipsilateral to the deprived eye in five cats and both ipsilateral and contralateral to the deprived eye in one cat). The percentages of X- and Y-cells encountered in the LGNd of our control animals is in agreement with previous estimates based on cell size (2:1 X- to Y-cells - Friedlander et al. 1981). In the present study, fewer normal X- and Y-cells were encountered in laminae innervated by the deprived eye than in laminae innervated by the non-deprived eye in every MD animal. When these values were normalized for the shrinkage of the deprived lamina, only the Y-cell population was significantly reduced. These differences are highly significant both when the data from all of the animals are pooled (chi 2 = 21.77; P less than 0.0001), and when the comparisons are made for individual animals (P less than 0.02, Mann-Whitney U-test). While some of the reduction in Y-cells may be due to an increase in the number of cells with abnormal receptive field properties, too few abnormal cells were encountered to totally account for the reduction in the number of Y-cells.