Kinetics of the subtransition in dipalmitoylphosphatidylcholine
The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solven...
Saved in:
Published in: | Biochemistry (Easton) 1987-07, Vol.26 (14), p.4288-4294 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-a476t-52d9dcfd319987f7381a18fcc4cb25b8c43861c8b1d5c790833dfb762c4190093 |
---|---|
cites | |
container_end_page | 4294 |
container_issue | 14 |
container_start_page | 4288 |
container_title | Biochemistry (Easton) |
container_volume | 26 |
creator | Tristram-Nagle, S Wiener, M. C Yang, C. P Nagle, J. F |
description | The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by others, DSC scans show two peaks in the subgel transition region for incubation temperatures below 1 degree C. After incubation at 0.1 degree C, the DSC peak that occurs at the lower scanning temperature appears with an incubation half-time of 0.5 day and eventually converts into a peak at higher scanning temperature with an incubation half-time of 18 days. By varying the scanning rate, we show that these two peaks merge into one at slow scanning rates with a common equilibrium transition temperature of 13.8 degrees C, in agreement with equilibrium calorimetry and dilatometry (delta V = 0.017 +/- 0.001 mL/g). For incubation temperatures above 4.6 degrees C, only one peak appears in both scanning dilatometry and calorimetry. While the initial rate of subgel conversion is smaller at the higher incubation temperatures, after 300 h a higher percentage of the sample has converted to subgel than at the lower incubation temperatures. We suggest that higher incubation temperatures (near 5 degrees C) are preferable for forming the stable subgel phase, and we present a colliding domain picture that indicates why this may be so. Our results in D2O and the similarity of the kinetics of volume decrease with the kinetics of wide-angle diffraction lines also support the suggestion that the partial loss of interlamellar water plays a kinetic role in subgel formation. |
doi_str_mv | 10.1021/bi00388a016 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81048754</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81048754</sourcerecordid><originalsourceid>FETCH-LOGICAL-a476t-52d9dcfd319987f7381a18fcc4cb25b8c43861c8b1d5c790833dfb762c4190093</originalsourceid><addsrcrecordid>eNptkE1r3DAQhkVJSDfbnnIO-BDSQ3E7sr5PIeym-WigC03PQpZlVqnXciwZuv--CrssOeQ0DO8zL8OD0BmGbxgq_L32AERKA5h_QDPMKiipUuwIzQCAl5Xi8BGdxvicVwqCnqATwjlhCmbo6qfvXfI2FqEt0toVcarTaProkw994fui8YPpNj6FbTesQxzWJvlm29l16PLpJ3Tcmi66z_s5R39-3Dwt7srHX7f3i-vH0lDBU8mqRjW2bQhWSopWEIkNlq211NYVq6WlRHJsZY0bZoUCSUjT1oJXlmIFoMgcXe56hzG8TC4mvfHRuq4zvQtT1BIDlYLRDH7dgXYMMY6u1cPoN2bcagz6VZd-oyvT5_vaqd645sDu_eT8Yp-baE3XZjPWxwMmOMs-X7Fyh_mY3L9DbMa_mgsimH5a_dYPy9VyhRd3epn5Lzve2KifwzT22d27D_4HYfWNZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81048754</pqid></control><display><type>article</type><title>Kinetics of the subtransition in dipalmitoylphosphatidylcholine</title><source>ACS CRKN Legacy Archives</source><creator>Tristram-Nagle, S ; Wiener, M. C ; Yang, C. P ; Nagle, J. F</creator><creatorcontrib>Tristram-Nagle, S ; Wiener, M. C ; Yang, C. P ; Nagle, J. F</creatorcontrib><description>The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by others, DSC scans show two peaks in the subgel transition region for incubation temperatures below 1 degree C. After incubation at 0.1 degree C, the DSC peak that occurs at the lower scanning temperature appears with an incubation half-time of 0.5 day and eventually converts into a peak at higher scanning temperature with an incubation half-time of 18 days. By varying the scanning rate, we show that these two peaks merge into one at slow scanning rates with a common equilibrium transition temperature of 13.8 degrees C, in agreement with equilibrium calorimetry and dilatometry (delta V = 0.017 +/- 0.001 mL/g). For incubation temperatures above 4.6 degrees C, only one peak appears in both scanning dilatometry and calorimetry. While the initial rate of subgel conversion is smaller at the higher incubation temperatures, after 300 h a higher percentage of the sample has converted to subgel than at the lower incubation temperatures. We suggest that higher incubation temperatures (near 5 degrees C) are preferable for forming the stable subgel phase, and we present a colliding domain picture that indicates why this may be so. Our results in D2O and the similarity of the kinetics of volume decrease with the kinetics of wide-angle diffraction lines also support the suggestion that the partial loss of interlamellar water plays a kinetic role in subgel formation.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00388a016</identifier><identifier>PMID: 3663590</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>1,2-Dipalmitoylphosphatidylcholine ; Artificial membranes and reconstituted systems ; Biological and medical sciences ; Calorimetry, Differential Scanning ; Fundamental and applied biological sciences. Psychology ; Gels ; Kinetics ; Membrane physicochemistry ; Molecular biophysics ; Molecular Conformation ; Thermodynamics</subject><ispartof>Biochemistry (Easton), 1987-07, Vol.26 (14), p.4288-4294</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a476t-52d9dcfd319987f7381a18fcc4cb25b8c43861c8b1d5c790833dfb762c4190093</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00388a016$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00388a016$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,27064,27924,27925,56766,56816</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7650740$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3663590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tristram-Nagle, S</creatorcontrib><creatorcontrib>Wiener, M. C</creatorcontrib><creatorcontrib>Yang, C. P</creatorcontrib><creatorcontrib>Nagle, J. F</creatorcontrib><title>Kinetics of the subtransition in dipalmitoylphosphatidylcholine</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by others, DSC scans show two peaks in the subgel transition region for incubation temperatures below 1 degree C. After incubation at 0.1 degree C, the DSC peak that occurs at the lower scanning temperature appears with an incubation half-time of 0.5 day and eventually converts into a peak at higher scanning temperature with an incubation half-time of 18 days. By varying the scanning rate, we show that these two peaks merge into one at slow scanning rates with a common equilibrium transition temperature of 13.8 degrees C, in agreement with equilibrium calorimetry and dilatometry (delta V = 0.017 +/- 0.001 mL/g). For incubation temperatures above 4.6 degrees C, only one peak appears in both scanning dilatometry and calorimetry. While the initial rate of subgel conversion is smaller at the higher incubation temperatures, after 300 h a higher percentage of the sample has converted to subgel than at the lower incubation temperatures. We suggest that higher incubation temperatures (near 5 degrees C) are preferable for forming the stable subgel phase, and we present a colliding domain picture that indicates why this may be so. Our results in D2O and the similarity of the kinetics of volume decrease with the kinetics of wide-angle diffraction lines also support the suggestion that the partial loss of interlamellar water plays a kinetic role in subgel formation.</description><subject>1,2-Dipalmitoylphosphatidylcholine</subject><subject>Artificial membranes and reconstituted systems</subject><subject>Biological and medical sciences</subject><subject>Calorimetry, Differential Scanning</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Kinetics</subject><subject>Membrane physicochemistry</subject><subject>Molecular biophysics</subject><subject>Molecular Conformation</subject><subject>Thermodynamics</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNptkE1r3DAQhkVJSDfbnnIO-BDSQ3E7sr5PIeym-WigC03PQpZlVqnXciwZuv--CrssOeQ0DO8zL8OD0BmGbxgq_L32AERKA5h_QDPMKiipUuwIzQCAl5Xi8BGdxvicVwqCnqATwjlhCmbo6qfvXfI2FqEt0toVcarTaProkw994fui8YPpNj6FbTesQxzWJvlm29l16PLpJ3Tcmi66z_s5R39-3Dwt7srHX7f3i-vH0lDBU8mqRjW2bQhWSopWEIkNlq211NYVq6WlRHJsZY0bZoUCSUjT1oJXlmIFoMgcXe56hzG8TC4mvfHRuq4zvQtT1BIDlYLRDH7dgXYMMY6u1cPoN2bcagz6VZd-oyvT5_vaqd645sDu_eT8Yp-baE3XZjPWxwMmOMs-X7Fyh_mY3L9DbMa_mgsimH5a_dYPy9VyhRd3epn5Lzve2KifwzT22d27D_4HYfWNZw</recordid><startdate>19870714</startdate><enddate>19870714</enddate><creator>Tristram-Nagle, S</creator><creator>Wiener, M. C</creator><creator>Yang, C. P</creator><creator>Nagle, J. F</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870714</creationdate><title>Kinetics of the subtransition in dipalmitoylphosphatidylcholine</title><author>Tristram-Nagle, S ; Wiener, M. C ; Yang, C. P ; Nagle, J. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a476t-52d9dcfd319987f7381a18fcc4cb25b8c43861c8b1d5c790833dfb762c4190093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>1,2-Dipalmitoylphosphatidylcholine</topic><topic>Artificial membranes and reconstituted systems</topic><topic>Biological and medical sciences</topic><topic>Calorimetry, Differential Scanning</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Kinetics</topic><topic>Membrane physicochemistry</topic><topic>Molecular biophysics</topic><topic>Molecular Conformation</topic><topic>Thermodynamics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tristram-Nagle, S</creatorcontrib><creatorcontrib>Wiener, M. C</creatorcontrib><creatorcontrib>Yang, C. P</creatorcontrib><creatorcontrib>Nagle, J. F</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tristram-Nagle, S</au><au>Wiener, M. C</au><au>Yang, C. P</au><au>Nagle, J. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of the subtransition in dipalmitoylphosphatidylcholine</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1987-07-14</date><risdate>1987</risdate><volume>26</volume><issue>14</issue><spage>4288</spage><epage>4294</epage><pages>4288-4294</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by others, DSC scans show two peaks in the subgel transition region for incubation temperatures below 1 degree C. After incubation at 0.1 degree C, the DSC peak that occurs at the lower scanning temperature appears with an incubation half-time of 0.5 day and eventually converts into a peak at higher scanning temperature with an incubation half-time of 18 days. By varying the scanning rate, we show that these two peaks merge into one at slow scanning rates with a common equilibrium transition temperature of 13.8 degrees C, in agreement with equilibrium calorimetry and dilatometry (delta V = 0.017 +/- 0.001 mL/g). For incubation temperatures above 4.6 degrees C, only one peak appears in both scanning dilatometry and calorimetry. While the initial rate of subgel conversion is smaller at the higher incubation temperatures, after 300 h a higher percentage of the sample has converted to subgel than at the lower incubation temperatures. We suggest that higher incubation temperatures (near 5 degrees C) are preferable for forming the stable subgel phase, and we present a colliding domain picture that indicates why this may be so. Our results in D2O and the similarity of the kinetics of volume decrease with the kinetics of wide-angle diffraction lines also support the suggestion that the partial loss of interlamellar water plays a kinetic role in subgel formation.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>3663590</pmid><doi>10.1021/bi00388a016</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0006-2960 |
ispartof | Biochemistry (Easton), 1987-07, Vol.26 (14), p.4288-4294 |
issn | 0006-2960 1520-4995 |
language | eng |
recordid | cdi_proquest_miscellaneous_81048754 |
source | ACS CRKN Legacy Archives |
subjects | 1,2-Dipalmitoylphosphatidylcholine Artificial membranes and reconstituted systems Biological and medical sciences Calorimetry, Differential Scanning Fundamental and applied biological sciences. Psychology Gels Kinetics Membrane physicochemistry Molecular biophysics Molecular Conformation Thermodynamics |
title | Kinetics of the subtransition in dipalmitoylphosphatidylcholine |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T16%3A19%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Kinetics%20of%20the%20subtransition%20in%20dipalmitoylphosphatidylcholine&rft.jtitle=Biochemistry%20(Easton)&rft.au=Tristram-Nagle,%20S&rft.date=1987-07-14&rft.volume=26&rft.issue=14&rft.spage=4288&rft.epage=4294&rft.pages=4288-4294&rft.issn=0006-2960&rft.eissn=1520-4995&rft_id=info:doi/10.1021/bi00388a016&rft_dat=%3Cproquest_cross%3E81048754%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a476t-52d9dcfd319987f7381a18fcc4cb25b8c43861c8b1d5c790833dfb762c4190093%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=81048754&rft_id=info:pmid/3663590&rfr_iscdi=true |