Methylation Increases Sodium Transport into A6 Apical Membrane Vesicles: Possible Mode of Aldosterone Action

When isolated apical membrane vesicles prepared from cultured A6 epithelia were incubated in vitro with the methyl donor S-adenosylmethionine, the control rate of amiloride-inhibitable sodium transport was doubled. The methylation inhibitors 3-deazaadenosine and S-adenosyl homocysteine returned the...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1984-08, Vol.225 (4663), p.745-746
Main Authors: Sariban-Sohraby, Sarah, Burg, Maurice, Wiesmann, William P., Chiang, Peter K., Johnson, John P.
Format: Article
Language:English
Subjects:
Aldosterone
Aldosterone - physiology
Aldosterone assay
Amiloride - pharmacology
Amphibians
Animals
Biological and medical sciences
Biological Transport, Active - drug effects
Cell Line
Cell lines
Cell Membrane - drug effects
Cell Membrane - metabolism
Cell membranes
epithelium
Fundamental and applied biological sciences. Psychology
kidney
Kidney - metabolism
Kidney cells
Lipids
Methylation
P branes
Phospholipids
S-Adenosylhomocysteine - pharmacology
S-Adenosylmethionine - pharmacology
Sodium
Sodium - metabolism
Steroid hormones. Cholecalciferol derivatives
String theory
Tubercidin - pharmacology
Vertebrates: endocrinology
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Summary:When isolated apical membrane vesicles prepared from cultured A6 epithelia were incubated in vitro with the methyl donor S-adenosylmethionine, the control rate of amiloride-inhibitable sodium transport was doubled. The methylation inhibitors 3-deazaadenosine and S-adenosyl homocysteine returned the S-adenosylmethionine-stimulated sodium transport to control levels. Neither these agents nor adenosine affected sodium transport into control vesicles. In vesicles incubated with S-adenosyl-[$^{3}$H-methyl]methionine, both membrane phospholipids and proteins were labeled, and this labeling was inhibited by deazaadenosine. In vesicles prepared from A6 cells treated with aldosterone, sodium transport was twice the control value and S-adenosylmethionine did not cause any further stimulation of transport. In those vesicles, both lipid and protein methylation were increased. These results suggest that methylation, which increases the rate of amiloride-sensitive sodium transport, is involved in the action of aldosterone at the apical membrane level in epithelia.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.6463652